Q. Subhani, Nadeem Muhammad, Zhouman Huang
Aug 25, 2020
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Influential Citations
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Journal
Journal of separation science
Abstract
This study aims to introduce a simple, sensitive, and cost-effective method for the simultaneous determination of acetamiprid and its main metabolite 6-chloronicotinic acid in environmental samples by using a non-suppressed ion chromatography hyphenated with an online post-column photoinduced fluorescence detection system. The fluorescence detector wavelengths λex / λem = 257/382 nm was set for up to 6.0 min for acetamiprid, while λex / λem = 231/370 nm programmed for 6-chloronicotinic acid for the rest of the analysis time. Both samples were treated by applying miniaturized quick, easy, cheap, effective, rugged, and safe method before the separation of analytes on an IonPac® AS11-HC column by pumping 40 mM NaOH having minuscule content of acetonitrile (5%, V/V) as an eluent. Both intrinsically non-fluorescent analytes were turned-on by online post-column photoinduced derivatization, avoiding the need for complex chemical derivatization or addition of a post-column extra pump. The developed method was appraised for the analysis of environmental samples, exhibiting excellent linearity (0.050 - 10 μg/mL) with a correlation coefficient greater than 0.9993 for both analytes. Whereas, obtained limit of detection (0.025 - 0.0072 μg/mL), recoveries (98.02 - 116.00 %), and inter- and intra-day precision (≤ 3.02 %) were satisfactory for both compounds in environmental samples. This article is protected by copyright. All rights reserved.