M. Green, M. Green, K. Tsou
Aug 1, 1955
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0
Influential Citations
183
Citations
Quality indicators
Journal
Archives of Biochemistry and Biophysics
Abstract
Abstract The synthesis of l -leucine-β-naphthylamide hydrochloride as a colorimetric substrate for the determination of leucine aminopeptidase is described along with the synthesis of optical isomers and other derivatives designed to clarify the specificity of the substrate. β-Naphthylamine, the product of enzymatic hydrolysis of the substrate, was determined either by a direct coupling method or by the use of the Bratton-Marshall procedure. The kinetics of the reaction were investigated and evidence for the specificity of the substrate was obtained. The distribution of enzymatic activity in mammalian tissue was investigated. A method was developed for the determination of leucine aminopeptidase in serum. The sera from pregnant women showed abnormally high values.