Zi Yan-qin
2004
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Journal
Journal of Huaibei Industry Teachers College
Abstract
The binding reaction of RNA (Ribonucleic Acid) with dye ethyl violet has been studied. In the medium of pH range 5.0~6.0,ethyl violet showed a decr ease in absorption at the wavelength of its maximal absorption peak(595 nm) on b inding to RNA.A novel method for the determination of RNA at 595 nm using ethyl violet as a labeling agent was developed. The method is of high sensitivity as w ell as selectivity ,and it has been used to determination RNA in samples with sa tisfactory results.