Shaista Tahir, A. Badshah, Raja Azadar Hussain
Nov 5, 2015
Citations
0
Influential Citations
26
Citations
Journal
Journal of Molecular Structure
Abstract
Abstract Four new nitrosubstituted acylthioureas i.e. 1-acetyl-3-(4-nitrophenyl)thiourea (TU1), 1-acetyl-3-(2-methyl-4-nitrophenyl)thiourea (TU2), 1-acetyl-3-(2-methoxy-4-nitrophenyl)thiourea (TU3) and 1-acetyl-3-(4-chloro-3-nitrophenyl)thiourea (TU4) have been synthesized and characterized (by C 13 and H 1 nuclear magnetic resonance, Fourier transform infrared spectroscopy and single crystal X-ray diffraction). As a preliminary investigation of the anti-cancer potencies of the said compounds, DNA interaction studies have been carried out using cyclic voltammetry and UV–vis spectroscopy along with verification from computational studies. The drug-DNA binding constants are found to be in the order, K TU3 9.04 × 10 6 M −1 > K TU4 8.57 × 10 6 M −1 > K TU2 6.05 × 10 6 M −1 > K TU1 1.16 × 10 6 M −1 . Furthermore, the antioxidant, cytotoxic, antibacterial and antifungal activities have been carried out against DPPH (1,1-diphenyl-2-dipicrylhydrazyl), Brine shrimp eggs, gram positive ( Micrococcus luteus , Staphylococcus aureus ) and gram negative ( Bordetella bronchiseptica , Salmonella typhimurium , Enterobacter aerogens ) and fungal cultures ( Aspergillus fumigatus , Mucor species, Aspergillus niger , Aspergillus flavus ) respectively.