Dodecyl gallate induces apoptosis by upregulating the caspase-dependent apoptotic pathway and inhibiting the expression of anti-apoptotic Bcl-2 family proteins in human osteosarcoma cells

doi:10.3892/mmr.2015.4717

Paper

Dodecyl gallate induces apoptosis by upregulating the caspase-dependent apoptotic pathway and inhibiting the expression of anti-apoptotic Bcl-2 family proteins in human osteosarcoma cells

Published Dec 24, 2015 · Chun-Hsiang Cheng, Yen-Po Cheng, I. Chang

Molecular Medicine Reports

Q2 SJR score

19

Citations

0

Influential Citations

Full textSemantic Scholar

Abstract

Dodecyl gallate (DG) is a gallic acid ester that has been shown to inhibit tumor growth. The aim of this study was to investigate the mechanism by which DG induces antiproliferative and apoptotic effects in MG-63 human osteosarcoma cells. Dose- and time-dependent cytotoxic effects of DG were determined using an MTT assay. The results showed that the half-maximal inhibitory concentration (IC50) of DG in MG-63 cells was 31.15 µM at 24 h, 10.66 µM at 48 h, and 9.06 µM at 72 h. Flow cytometric analysis demonstrated that exposure to 20 and 40 µM DG resulted in an increase in the sub-G1 phase population and in S-phase cell cycle arrest. Furthermore, western blot analysis of apoptosis-related protein expression revealed an increase in the activation of caspases 8 and 3, cleavage of poly (ADPribose) polymerase (PARP), and disruption of mitochondrial membrane permeability was measured by flow cytometry. An increase in the Bax/Bcl-2 ratio and a decrease in the expression of inhibitor of apoptosis protein (IAP) family members, namely X-linked inhibitor of apoptosis protein and survivin, were also observed following DG treatment. These data provide insight into the molecular mechanisms governing the ability of DG to induce apoptosis in human osteosarcoma cells in vitro.

In Vitro Study

Study Snapshot

Dodecyl gallate induces apoptosis in human osteosarcoma cells by upregulating the caspase-dependent apoptotic pathway and inhibiting anti-apoptotic Bcl-2 family proteins.

PopulationOlder adults (50-71 years)

Sample size24

MethodsObservational

OutcomesBody Mass Index projections

ResultsSocial networks mitigate obesity in older groups.

Sign up to use Study Snapshot

Consensus is limited without an account. Create an account or sign in to get more searches and use the Study Snapshot.

Create an account

Paper

Dodecyl gallate induces apoptosis by upregulating the caspase-dependent apoptotic pathway and inhibiting the expression of anti-apoptotic Bcl-2 family proteins in human osteosarcoma cells

Sign up to use Study Snapshot

References

Citations

Remimazolam Suppresses Oxidative Stress and Apoptosis in Cerebral Ischemia/Reperfusion Injury by Regulating AKT/GSK-3β/NRF2 Pathway

Remimazolam activates the AKT/GSK-3/NRF2 pathway, reducing oxidative stress and apoptosis in brain ischemia/reperfusion injury.

Inhibitory Effects of Soluble Dietary Fiber from Foxtail Millet on Colorectal Cancer by the Restoration of Gut Microbiota.

Foxtail millet soluble dietary fiber (FMB-SDF) significantly alleviates colon cancer symptoms in mice by restoring gut microbiota diversity and regulating cancer-related signaling pathways.

The role of dietary polyphenols in osteosarcoma: A possible clue about the molecular mechanisms involved in a process that is just in its infancy.

Dietary polyphenols show potential in osteosarcoma treatment due to their health-promoting properties, but more human studies are needed to validate their effectiveness and their interactions with chemotherapeutic drugs.

ICT1 Promotes Osteosarcoma Cell Proliferation and Inhibits Apoptosis via STAT3/BCL-2 Pathway

ICT1 promotes osteosarcoma cell growth and inhibits apoptosis through the STAT3/BCL-2 pathway, offering potential targets for novel treatments.

Alkyl and Aryl Derivatives Based on p-Coumaric Acid Modification and Inhibitory Action against Leishmania braziliensis and Plasmodium falciparum

Hexyl p-coumarate derivative (9) shows the highest leishmanicidal potency against Leishmania braziliensis, while methyl p-coumarate demonstrates antiplasmodial activity against Plasmodium falciparum.

Synergistic Effects of Lauryl Gallate and Tamoxifen on Human Breast Cancer Cell

The combination of LG and TAM is more effective in inducing apoptosis of breast cancer cells compared to individual use of each drug, offering new therapeutic options for suppressing breast cancer growth.