A novel effect of the new antileukemic drug, 2-chloro-2'-deoxyadenosine, in human lymphocytes.

doi:10.1006/BBRC.1994.2337

Paper

A novel effect of the new antileukemic drug, 2-chloro-2'-deoxyadenosine, in human lymphocytes.

Published Sep 30, 1994 · M. Sasvári-Székely, Z. Piróth, Z. Kazimierczuk

Biochemical and biophysical research communications

Q1 SJR score

14

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0

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Abstract

2-chloro-2'-deoxyadenosine (Cl-dAdo) is a potent drug against hairy cell leukemia and other lymphomas. Its effects on the metabolism of labeled deoxycytidine (5-[3H]dCyd) and deoxythymidine ([3H]dThd) were investigated in short term cultures of human lymphocytes. In the presence of Cl-dAdo, a more pronounced inhibition of DNA synthesis was found, accompanied with a much higher accumulation of radioactivity in dTTP than in dCTP. This difference disappeared in the presence of exogenous dThd. Analysis of intracellular metabolites of 5-[3H]dCyd showed an increase in [3H]dCTP used for liponucleotide biosynthesis and a decrease in [3H]dUMP formation in the presence of Cl-dAdo, suggesting the possible inhibition of dCMP deaminase.

In Vitro Study

Study Snapshot

The new antileukemic drug, 2-chloro-2'-deoxyadenosine, inhibits DNA synthesis in human lymphocytes, potentially inhibiting dCMP deaminase.

PopulationOlder adults (50-71 years)

Sample size24

MethodsObservational

OutcomesBody Mass Index projections

ResultsSocial networks mitigate obesity in older groups.

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Paper

A novel effect of the new antileukemic drug, 2-chloro-2'-deoxyadenosine, in human lymphocytes.

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References

Deoxycytidine is salvaged not only into DNA but also into phospholipid precursors.

Deoxycytidine metabolism in human tonsillar lymphocytes involves incorporation into phospholipid precursors, including dCDP-choline and dCDP-ethanolamine, in addition to DNA synthesis.

Citations