J. L. Mego
Mar 30, 1964
Citations
3
Influential Citations
49
Citations
Quality indicators
Journal
Biochimica et biophysica acta
Abstract
Abstract 1. 1. Hadacidin, an inhibitor of adenine synthesis, is an effective inhibitor of chloroplast formation in non-dividing, dark-grown Euglena cells. The degree inhibition is proportional to the concentration of hadacidin in the medium, and the inhibition is relieved by adenine or aspartic acid. These observations are consistent with the work of Shigeura and Gordon 3 in which these authors implicated the enzyme IMP: L -aspartate ligase (EC 6.3.4.4) as the site of action of hadacidin. 2. 2. Hadacidin has no effect on further chlorophyll formation when added after 24 h of light and only 16% of the total chlorophyll has been formed. The addition of hadacidin after 1 h of light results in more chlorophyll formation than in cultures added at zero time. These results suggests that the synthesis of the adenine involved in chloroplast development begins within 1 h after exposure to light and is complete within 24 h.