On the metabolic effects of 6-chloropurine.

Paper

On the metabolic effects of 6-chloropurine.

Published Sep 1, 1961 · D. E. Duggan, M. Weigert, E. Titus

Cancer research

Q1 SJR score

7

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0

Influential Citations

Semantic Scholar

Abstract

Summary 6-Chloropurine and 6-chlorouric acid effect specific inhibitions of xanthine oxidase and uricase, respectively, in both slices and homogenates of rat liver. In the rat in vivo , a similarly potent inhibition of uricase is achieved by 6-chlorouric acid, whether administered as such or formed in vivo by the oxidation of 6-chloropurine. 6-Chloropurine in vivo and in liver slices effects only a slight inhibition of nucleic acid biosynthesis as measured by the incorporation of glycine-2-C 14 and of P 32 . The utilization of acetate for lipide synthesis and the incorporation of glycine in protein are similarly decreased to only a small extent in the presence of therapeutic levels of the drug.

In Vitro Study

Study Snapshot

6-chloropurine and 6-chlorouric acid inhibit xanthine oxidase and uricase in rat liver, with 6-chloropurine showing slightly less inhibition of nucleic acid biosynthesis and acetate utilization.

PopulationOlder adults (50-71 years)

Sample size24

MethodsObservational

OutcomesBody Mass Index projections

ResultsSocial networks mitigate obesity in older groups.

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Paper

On the metabolic effects of 6-chloropurine.

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References

A simple efficient liquid scintillator for counting aqueous solutions in a liquid scintillation counter

The modified naphthalene-dioxane-PPO liquid scintillator efficiently counts up to 3.0 ml of aqueous solutions, with a 65.6% efficiency for carbon-14 and 11.7% efficiency for tritiated water.

Inhibition of the biosynthesis of purines de novo by 6-chloropurine and azaserine in sensitive and naturally resistant ascites cells.

Combinations of 6-chloropurine and azaserine effectively inhibit purine biosynthesis in sensitive ascites-cell neoplasms, potentially affecting guanine formation in resistant tumors.

Modifications in purine metabolism induced by 6-chloropurine.

6-chloropurine inhibits glycine-2-C 14 utilization for nucleic acid guanine formation in Sarcoma 180 ascites cells, suggesting a compensatory reaction during decreased guanine de novo synthesis.

Studies on the mechanism of action of 6-mercaptopurine in sensitive and resistant L1210 leukemia in vitro.

6-mercaptopurine inhibits nucleic acid metabolism in L1210 leukemia cells, causing a metabolic block in the conversion of inosinic acid to adenylic acid, with limited capacity for conversion in resistant cells.

6-Chloropurine and 6-chlorouric acid as substrates and inhibitors of purine-oxidizing enzymes.

6-chloropurine possesses carcinostatic properties and is a useful intermediate in synthesis of other purines, offering potential for converting trace quantities of starting drugs and metabolites to known compounds.

The effects of 8-azaguanine and 6-mercaptopurine on purine catabolism in the rat.

Pharmacologic doses of 8-azaguanine and 6-mercaptopurine inhibit xanthine catabolism in vivo in rats, suggesting xanthine oxidase is a rate-limiting reaction in purine metabolism.

A simple method for the isolation and purification of total lipides from animal tissues.

The simplified version of the method for preparing and purifying brain lipides from animal tissues reduces lipide losses and produces a more efficient extraction of non-lipide substances.

Citations