Hypotensive Effects and Angiotensin-Converting Enzyme Inhibitory Peptides of Reishi (Ganoderma lingzhi) Auto-Digested Extract

doi:10.3390/molecules190913473

Paper

Hypotensive Effects and Angiotensin-Converting Enzyme Inhibitory Peptides of Reishi (Ganoderma lingzhi) Auto-Digested Extract

Published Aug 29, 2014 · Hai-Bang Tran, A. Yamamoto, Sayaka Matsumoto

Molecules

Q1 SJR score

35

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1

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Abstract

Reishi (Ganoderma lingzhi) has been used as a traditional medicine for millennia. However, relatively little is known about this mushroom’s proteins and their bioactivities. In this study, we used reishi’s own proteases to hydrolyze its protein and obtained auto-digested reishi (ADR) extract. The extract was subjected to in vitro assays and administered to spontaneous hypertensive rats (SHRs) to determine its potential for use as a hypotensive medication. Bioassay-guided fractionation and de novo sequencing were used for identifying the active compounds. After 4 h administration of ADR, the systolic pressure of SHRs significantly decreased to 34.3 mmHg (19.5% change) and the effect was maintained up to 8 h of administration, with the decrease reaching as low as 26.8 mmHg (15% reduction–compare to base line a decrease of 26.8 mmHg is less than a decrease of 34.3 mmHg so it should give a smaller % reduction). Eleven peptides were identified and four of them showed potent inhibition against ACE with IC50 values ranging from 73.1 μM to 162.7 μM. The results showed that ADR could be a good source of hypotensive peptides that could be used for antihypertensive medication or incorporation into functional foods.

In Vitro Study

Study Snapshot

Auto-digested reishi extract significantly reduces systolic pressure in hypertensive rats and contains four peptides with potent inhibition against angiotensin-converting enzyme, making it a potential source for antihypertensive medication or functional foods.

PopulationOlder adults (50-71 years)

Sample size24

MethodsObservational

OutcomesBody Mass Index projections

ResultsSocial networks mitigate obesity in older groups.

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Paper

Hypotensive Effects and Angiotensin-Converting Enzyme Inhibitory Peptides of Reishi (Ganoderma lingzhi) Auto-Digested Extract

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References

Novel angiotensin I-converting enzyme inhibitory peptides derived from edible mushroom Agaricus bisporus (J.E. Lange) Imbach identified by LC-MS/MS.

AHEPVK, RIGLF, and PSSNK are potent angiotensin I-converting enzyme inhibitory peptides from edible mushrooms Agaricus bisporus, making them ideal ingredients for antihypertensive supplements.

Wild Mushrooms in Nepal: Some Potential Candidates as Antioxidant and ACE-Inhibition Sources

Nepalese wild mushrooms show high antioxidant and ACE-inhibitory properties, with Phlebia tremellosa showing potential as an antihypertensive agent.

Novel angiotensin I-converting enzyme inhibitory peptides derived from an edible mushroom, Pleurotus cystidiosus O.K. Miller identified by LC-MS/MS

Peptides from Pleurotus cystidiosus edible mushrooms show potential as ACE inhibitors, with lower activity compared to commercial antihypertensive drugs but no side effects.

Proteomic analysis of antihypertensive proteins in edible mushrooms.

Antihypertensive activity in edible mushrooms Pleurotus cystidiosus and Agaricus bisporus could be due to proteins with molecular masses ranging from 3 to 10 kDa.

Bioactive peptides: are there more antihypertensive mechanisms beyond ACE inhibition?

Bioactive peptides from various food sources show antihypertensive effects beyond ACE inhibition, with potential mechanisms including phosphodiesterase inhibition, nitric oxide production, and nitric oxide synthase inhibition.

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