F. Guo, Xiaoxue Wang, Xinxin Liu
Oct 29, 2020
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Influential Citations
8
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Quality indicators
Journal
Phytotherapy Research
Abstract
The rhizome of Belamcanda chinensis possesses antiinflammatory and antioxidant activities. However, the effect of irigenin, isolated from the rhizome of B. chinensis, on 1‐methyl‐4‐phenylpyridinium (MPP+)‐induced neurotoxicity is unknown. MTT assay showed that MPP+ exposure dose dependently inhibited the viability of mouse microglia BV‐2 cells, whereas irigenin suppressed MPP+‐induced viability reduction. The production of nitric oxide, prostaglandin E2, tumor necrosis factor‐α and interleukin‐6 were increased by MPP+ treatment, which were abolished by irigenin treatment. Irigenin‐attenuated MPP+‐induced increase of malondialdehyde content and activities of superoxide dismutase, catalase and glutathione peroxidase in BV‐2 cells. Irigenin treatment also repressed apoptosis, caspase‐3/7 activity and Cytochrome C expression in MPP+‐challenged BV‐2 cells. Interestingly, irigenin activated the Keap1/Nrf2 pathway in MPP+‐induced BV‐2 cells. Nrf2 knockdown attenuated the effects of irigenin on MPP+‐induced viability reduction, inflammation, oxidative stress and apoptosis in BV‐2 cells. In conclusion, irigenin alleviated MPP+‐induced neurotoxicity in BV‐2 cells through regulating the Keap1/Nrf2 pathway.