M. S. Akhtar, A. K. Pal, N. P. Sahu
May 1, 2012
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Influential Citations
24
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Quality indicators
Journal
Pesticide Biochemistry and Physiology
Abstract
Abstract A sixty-day experiment was carried out to study the effect of dietary pyridoxine (PN) on growth performance, RNA/DNA ratio and some biochemical parameters of Labeo rohita fingerlings exposed to sub-lethal dose of endosulfan (1/10th of 96 h static non-renewal LC 50 = 0.2 ppb) to assess the role of pyridoxine in ameliorating the negative effects of endosulfan. Two hundred seventy fingerlings (6.5 ± 0.26 g) were randomly distributed into six treatments in triplicates (15 fish/tank). Five iso-nitrogenous (35.45–35.75% crude protein) purified diets were prepared with graded levels of pyridoxine. Six treatment groups were T 0 (10 mg PN + without endosulfan), T 1 (0 mg PN + endosulfan), T 2 (10 mg PN + endosulfan), T 3 (50 mg PN + endosulfan), T 4 (100 mg PN + endosulfan) and T 5 (200 mg PN + endosulfan). Weight gain (%), specific growth rate (SGR), tissue glycogen, and protease activity were significantly ( P R 2 = 0.931) with (%) weight gain. Glucose-6-phosphate dehydrogenase (G6PDH) activity was significantly ( P P > 0.05) effect of dietary pyridoxine on feed conversion ratio (FCR), protein efficiency ratio (PER), survival, gastro-somatic index (GSI), hepato-somatic index (HSI) and liver and muscle DNA levels of L. rohita fingerlings. RNA levels, both in liver and muscle, increased significantly ( P R 2 = 0.91) and muscle ( R 2 = 0.88). RNA/DNA ratio showed a third order polynomial relationship with dietary pyridoxine, both in liver ( Y = −0.014 x 3 + 0.1613 x 2 − 0.5333 x + 0.7933, R 2 = 0.987) and muscle ( Y = −0.0407 x 3 + 0.4763 x 2 − 1.6358 x + 2.4667, R 2 = 0.9345). The overall results obtained in present study indicated that dietary pyridoxine supplementation at 100 or 200 mg PN/kg diet ameliorates the negative effects of endosulfan and restores optimal growth of L. rohita fingerlings.