The effects of pyroglutamylglutamylprolineamide, a peptide related to thyrotrophin-releasing hormone, on rat anterior pituitary cells in culture.

doi:10.1677/JOE.0.1420111

Paper

The effects of pyroglutamylglutamylprolineamide, a peptide related to thyrotrophin-releasing hormone, on rat anterior pituitary cells in culture.

Published Jul 1, 1994 · R. Ashworth, J. Ham, S. M. Cockle

The Journal of endocrinology

Q1 SJR score

11

Citations

0

Influential Citations

Full textSemantic Scholar

Abstract

Pyroglutamylglutamylprolineamide, which was first discovered in mammalian prostate, differs from thyrotrophin-releasing hormone (TRH) by substitution of glutamic acid for histidine at position two of the tripeptide. Recently, the newly discovered peptide has been identified in substantial concentrations in the rat anterior pituitary gland and, in this study, we have investigated the effects of the peptide on rat anterior pituitary cells in culture. GH3 cells were chosen to examine the possible effects of the new peptide, particularly in relation to its effects on the TRH receptor. This cell-type was deficient, in comparison with normal rat pituitary cells, in the new TRH-related peptide and appeared to be an ideal model cell in which to study the effects of pGlu-Glu-ProNH2. TRH (0.01-100 nM) was found to stimulate the secretion of both GH and prolactin from GH3 cells whereas pGlu-Glu-ProNH2 had no effect within the same concentration ranges. In contrast, at micromolar concentrations pGlu-Glu-ProNH2 exhibited intrinsic TRH-like activity causing stimulation of both GH and prolactin release from GH3 cells. Both TRH and pGlu-Glu-ProNH2 appeared to act through the same intracellular signalling mechanism, causing significant increases in intracellular inositol phosphate within the expected concentration ranges. However, pGlu-Glu-ProNH2 (up to 1 mM) displaced neither [3H]TRH nor [3H]MeTRH from membrane-binding sites on GH3 cells, suggesting that the effects of the new peptide were mediated through a second receptor. The physiological relevance of these effects of pGlu-Glu-ProNH2 requires further investigation.

In Vitro Study

Study Snapshot

Pyroglutamylglutamylprolineamide (pGlu-Glu-ProNH2) stimulates GH and prolactin secretion from rat anterior pituitary cells, suggesting its potential physiological relevance.

PopulationOlder adults (50-71 years)

Sample size24

MethodsObservational

OutcomesBody Mass Index projections

ResultsSocial networks mitigate obesity in older groups.

Sign up to use Study Snapshot

Consensus is limited without an account. Create an account or sign in to get more searches and use the Study Snapshot.

Create an account

Paper

The effects of pyroglutamylglutamylprolineamide, a peptide related to thyrotrophin-releasing hormone, on rat anterior pituitary cells in culture.

Sign up to use Study Snapshot

References

Citations

Contrasting effects of A1 and A2b adenosine receptors on adipogenesis

A1 receptors stimulate adipogenesis, while A2b receptors inhibit adipogenesis and stimulate an osteoblastic phenotype, suggesting potential targets for obesity and diabetes management.

Human Osteoblast Precursors Produce Extracellular Adenosine, Which Modulates Their Secretion of IL‐6 and Osteoprotegerin

Human osteoblast precursors produce adenosine, which stimulates IL-6 secretion but inhibits osteoprotegerin secretion, suggesting adenosine as a new regulator of progenitor cell function.

Biological activity of activating thyroid-stimulating hormone receptor mutants depends on the cellular context.

Activating TSH receptor mutants' biological activity in thyroid cells depends on cellular context and expression levels, with differentiated functions more readily stimulated than growth, and their effects may be attenuated by complex counterregulatory mechanisms.

Adenosine-Regulated Cell Proliferation in Pituitary Folliculostellate and Endocrine Cells: Differential Roles for the A1 and A2B Adenosine Receptors.

Adenosine stimulates growth in pituitary folliculostellate cells but inhibits it in endocrine cells, highlighting differential expression of A(1) and A(2B) adenosine receptors in pituitary cells.

Adipogenesis in thyroid eye disease.

Orbital fat TSHR transcripts are expressed as functional protein, with most preadipocytes undergoing differentiation expressing the receptor, suggesting a key role for this population in increasing orbital volume in thyroid eye disease.