A. del Campillo-Campbell, D. Dykhuizen, P. Cleary
1979
Citations
2
Influential Citations
22
Citations
Journal
Methods in enzymology
Abstract
Publisher Summary This chapter describes the enzymic reduction of d-biotin d-sulfoxide to d-biotin. Biotin sulfoxides are ubiquitous in nature; they are present in the culture filtrates of various microorganisms and in cows' milk. The d-isomer is an oxidative product contaminating most preparations of authentic biotin. In an aerobic environment, biotin d-sulfoxide may be one of the major chemical forms of biotin; and therefore, an important source of biotin for organisms to utilize this oxidized vitamer. The natural function of sulfoxide reduction may be either to scavenge biotin sulfoxide from the environment or else to reduce bound intracellular biotin that happens to become oxidized. The reduction of biotin d-sulfoxide to biotin is a complex reaction.