G. Sharma, B. Chakraborty, yotsana Sanadya
Jan 22, 2010
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Abstract
Losartan, is highly effective blood pressure lowering agent, which directly works by selectively blocking angiotensin-I (AT-I) receptor. It is primarily metabolized in active form, Losartan carboxylic acid (LCA). A sensitive and selective LC-MS/MS method for the determination of Losartan and Losartan Carboxylic Acid in human plasma was developed and validated. The ammonium formate: methanol (50:50 V/V) buffer was selected as the mobile phase; as the retention time was about only 1 minute and valsartan was taken as internal standard, due to resemble structural and chemical nature with the Losartan. The results were reproducible and precise with the samples prepared by solid phase extraction technique using “OASIS” HLB Cartridges (30mg), during method development trials; therefore it was used in sample preparation, for whole bioanalytical study of Losartan. The limit of quantification was from 25 ng /ml to 1500 ng/ml.