Mei-Liang Chin-Chen, S. Carda‐Broch, J. Peris-Vicente
Feb 1, 2013
Citations
0
Influential Citations
18
Citations
Journal
Journal of Food Composition and Analysis
Abstract
Abstract A simple, selective and sensitive method to quantify the biogenic amines cadaverine, 2-phenylethylamine, histamine and spermidine has been developed. The analytes were derivatized with 3,5-dinitrobenzoyl chloride and separated by micellar liquid chromatography. This is a practical technique for the selective determination and quantification of biogenic amines in fish sauce. Optimization of chromatographic conditions was made by an interpretative model, and the separation conditions were: C18 column (125 mm × 4.6 mm, 5 μm particle size), UV detection set at 260 nm, and a mobile phase of 0.15 mol L −1 sodium dodecyl sulfate (SDS), pH 7. Validation was performed following the United States Food and Drug Administration (FDA) guidelines using spiked samples. Under these conditions, validation parameters were: linearity (0.5–500 μg mL −1 , r 2 > 0.9990), limits of detection (in the 158–375 ng mL −1 range); intra and inter-day precision (relative standard deviation