A Ratiometric Fluorescent Probe for Monitoring Leucine Aminopeptidase in Living Cells and Zebrafish Model.

doi:10.1021/acs.analchem.7b02910

Paper

A Ratiometric Fluorescent Probe for Monitoring Leucine Aminopeptidase in Living Cells and Zebrafish Model.

Published Oct 26, 2017 · Zhe Zhou, Feiyi Wang, Guichun Yang

Analytical chemistry

Q1 SJR score

73

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Abstract

Leucine aminopeptidase (LAP) is an important cancer-related biomarker, which shows significant overexpression in malignant tumor cells like liver cancer. Developing an effective method to monitor LAP in tumor cells holds great potential for cancer diagnosis, treatment, and management. In this work, we report a novel BODIPY-based fluorescent probe (BODIPY-C-Leu) capable of monitoring LAP in vitro and in vivo in both ratiometric and turn-on model. BODIPY-C-Leu contains an asymmetrical BODIPY dye for fluorescent signaling and a dipeptide (Cys-Leu) as the triggered moiety. Activation occurs by cleavage of the amide bond in dipeptides and subsequently an intramolecular S → N conversion to convert sulfur-substituted BODIPY to amino-substituted BODIPY, resulting in a dramatic fluorescence variation to realize the detection of LAP. Furthermore, we have successfully employed BODIPY-C-Leu to monitor LAP activity in different cancer cells, indicating that HeLa cells have a higher level of LAP activity than A549 cells. Importantly, we demonstrated the capability of the probe for real-time monitoring the drug-induced LAP level changes in zebrafish.

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Study Snapshot

The BODIPY-C-Leu probe effectively monitors leucine aminopeptidase activity in cancer cells and zebrafish, offering potential for cancer diagnosis, treatment, and management.

PopulationOlder adults (50-71 years)

Sample size24

MethodsObservational

OutcomesBody Mass Index projections

ResultsSocial networks mitigate obesity in older groups.

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A Ratiometric Fluorescent Probe for Monitoring Leucine Aminopeptidase in Living Cells and Zebrafish Model.

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References

A fluorogenic 2D glycosheet for the simultaneous identification of human- and avian-receptor specificity in influenza viruses

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A novel coumarin-based water-soluble fluorescent probe for endogenously generated SO2 in living cells

The novel coumarin-based fluorescent probe SS-1 effectively detects and visualizes endogenously generated SO2 in living cells with low cytotoxicity.

Ultrasensitive Detection of Aminopeptidase N Activity in Urine and Cells with a Ratiometric Fluorescence Probe.

Ultrasensitive ratiometric fluorescent probe (CVN) can detect aminopeptidase N activity in human urine and cells, aiding in diagnosis and understanding of APN-related diseases.

Luminescent Lanthanide Cyclen-Based Enzymatic Assay Capable of Diagnosing the Onset of Catheter-Associated Urinary Tract Infections Both in Solution and within Polymeric Hydrogels.

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Enzymatic Cleavage and Subsequent Facile Intramolecular Transcyclization for in Situ Fluorescence Detection of γ-Glutamyltranspetidase Activities.

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In Situ Ratiometric Quantitative Tracing of Intracellular Leucine Aminopeptidase Activity via an Activatable Near-Infrared Fluorescent Probe.

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Detection of Intracellular Selenol-Containing Molecules Using a Fluorescent Probe with Near-Zero Background Signal.

This new fluorogenic molecular probe is highly specific and sensitive for detecting intracellular selenols, aiding in clinical diagnosis for human diseases associated with selenium deficiency or overdose.

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