Alfred Tuley, Yan-Jiun Lee, Bo Wu
Jun 17, 2014
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0
Influential Citations
29
Citations
Journal
Chemical communications
Abstract
Using a mutant pyrrolysyl-tRNA synthetase-tRNA(Pyl)(CUA) pair, 3-formyl-phenylalanine is genetically incorporated into proteins at amber mutation sites in Escherichia coli. This non-canonical amino acid readily reacts with hydroxylamine dyes, leading to rapid and site-selective protein labelling.