Growth inhibition of human colon cancer cell line HCT116 by bis[2-(acylamino)phenyl] disulfide and its action mechanism.

doi:10.1248/BPB.31.916

Paper

Growth inhibition of human colon cancer cell line HCT116 by bis[2-(acylamino)phenyl] disulfide and its action mechanism.

Published May 1, 2008 · Satoshi Yamakawa, Aya Demizu, Y. Kawaratani

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Abstract

Our laboratory has been investigating the use of compounds which disrupt beta-catenin/T cell factor (TCF) binding to treat human colon cancer. There are several cysteine residues on the surface of beta-catenin where it binds to TCF. Some bis[2-(acylamino)phenyl] disulfides might have the ability to form a disulfide bond with the cysteine residues of beta-catenin, leading to inhibition of the growth of human colon cells. Bis[2-(acylamino)phenyl] disulfides were screened to inhibit the growth of cancer cells. Among them, bis[2-(2,2-dimethylpropanoylamino)phenyl] disulfide (1) had promising inhibitory effects (HCT116, IC50: 9.7 microM; DLD-1, IC50: 6.9 microM) on cell proliferation, and did not show any cytotoxicity among normal human fibroblast CCD-1059SK cells even at 200 microM. This derivative reduced the beta-catenin/TCF4 association in the HCT116 cells to ca. 50% at 150 microM. Furthermore, it activated markedly the phosphorylation of c-Jun N-terminal kinase (JNK) connected to stress-activated apoptosis at a lower concentration (30 microM). In view of cell cycle analyses, Hoechst staining, and terminal deoxynucleotidyl transferase-mediated dUTP-biotin Nick end-labeling (TUNEL) assays along with the above results, it is likely that 1 inhibited the growth of HCT116 cells through pathways including the JNK-mediated apoptosis.

In Vitro Study

Study Snapshot

Bis[2-(2-dimethylpropanoylamino)phenyl] disulfide (1) effectively inhibits human colon cancer cell growth through pathways including JNK-mediated apoptosis, without causing cytotoxicity in normal human fibroblasts.

PopulationOlder adults (50-71 years)

Sample size24

MethodsObservational

OutcomesBody Mass Index projections

ResultsSocial networks mitigate obesity in older groups.

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Growth inhibition of human colon cancer cell line HCT116 by bis[2-(acylamino)phenyl] disulfide and its action mechanism.

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References

Chemical insights in the concept of hybrid drugs: the antitumor effect of nitric oxide-donating aspirin involves a quinone methide but not nitric oxide nor aspirin.

The antitumor effect of hybrid drug 1 (NO-ASA) is solely based on a quinone methide, not nitric oxide or aspirin, with a derivative lacking these components being 10 times more effective than the original.

Cytotoxicity of lissoclibadins and lissoclinotoxins, isolated from a tropical ascidian Lissoclinum cf. badium, against human solid-tumor-derived cell lines.

Lissoclibadins 1 and 2 show potent inhibitory activity against human cancer cell lines, with potential anticancer efficacy, and show no toxicity after 50 mg/kg single treatment in mice.

Inhibition of protein–protein interactions: The discovery of druglike β‐catenin inhibitors by combining virtual and biophysical screening

Combining virtual and biophysical screening, we identified three druglike, low molecular weight Tcf4-competitive compounds, potentially useful for inhibiting protein-protein interactions in cancer.

The adenomatous polyposis coli protein (APC) exists in two distinct soluble complexes with different functions

The APC protein has two distinct functions, regulating -catenin and microtubules, and its distribution in cells is dynamic, allowing cells to allocate it as needed.

Potent histone deacetylase inhibitors: N-hydroxybenzamides with antitumor activities.

N-hydroxybenzamides with a 6-amino-2-naphthylcarbonyl group show promising antitumor activity and improved water solubility, with a maximum 185% survival rate in mice inoculated with P388 cells.

Citations

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