Hexadecylphosphocholine inhibits invasion of mouse T-cell lymphoma cells in two different invasion assays.

Paper

Hexadecylphosphocholine inhibits invasion of mouse T-cell lymphoma cells in two different invasion assays.

Published Mar 1, 1998 · H. Schaider, H. Seidl, M. Berger

Anticancer research

Q2 SJR score

3

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0

Influential Citations

Semantic Scholar

Abstract

Hexadecylphosphocholine (HePC), an ether lipid analogue, is a new antineoplastic drug which has been shown to exert a remarkable antiproliferative effect in vitro and in vivo. The signal transduction pathway and the phospholipid synthesis are thought to be the main putative molecular targets of HePC, yet the exact mechanism of action is still unclear. To investigate the antiinvasive activity of HePC on a mouse T-cell lymphoma cell line (BW-O-Li1), we used a type I collagen gel and devitalized dermis as substrate to evaluate the migration of BW-O-Li1 after exposure to HePC. BW-O-Li1 cells were exposed for 24 h to a non-cytotoxic (10 microM) as well as to cytotoxic concentrations of HePC. Afterwards, BW-O-Li1 cells were seeded on top of a reconstituted collagen gel layer or pippeted into a steel ring placed on the dermal site of a devitalized dermis. Lymphoma cells, which invaded the collagen layer were counted by light microscopy, invasion into devitalized dermis was measured by an image analysis system. Compared to unexposed cells, invasion into the collagen gel differed significantly even at 10 microM HePC, whereas the absolute number of invading cells, independently of the HePC concentration, showed no difference in the amount of counted cells. Migration into devitalized dermis was significantly reduced for 10 microM and 40 microM HePC. These data show that complementary information can be obtained by application of the two invasion assays and that the antiinvasive effect of HePC emerges at non-cytotoxic concentrations of the substance.

In Vitro Study

Study Snapshot

Hexadecylphosphocholine (HePC) shows antiinvasive activity against mouse T-cell lymphoma cells at non-cytotoxic concentrations, potentially benefiting cancer treatment.

PopulationOlder adults (50-71 years)

Sample size24

MethodsObservational

OutcomesBody Mass Index projections

ResultsSocial networks mitigate obesity in older groups.

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Paper

Hexadecylphosphocholine inhibits invasion of mouse T-cell lymphoma cells in two different invasion assays.

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References

Citations

Development of a quantitative method to analyse tumour cell invasion in organotypic culture

The developed 'Invasion Index' is a robust, accurate, and reproducible method for measuring tumor cell invasion in organotypic gel culture, allowing assessment of positive and negative regulation influences.

Overexpression of Diacylglycerol Acyltransferase-1 Reduces Phospholipid Synthesis, Proliferation, and Invasiveness in Simian Virus 40-transformed Human Lung Fibroblasts*

Overexpression of DGAT1 in human lung fibroblasts reduces phospholipid synthesis, cell growth rate, and invasiveness, potentially affecting cancer cell growth.