C. Compagno, A. Tura, B. M. Ranzi
2004
Citations
0
Influential Citations
3
Citations
Journal
Applied Microbiology and Biotechnology
Abstract
SummaryOne of the methods commonly used for manufacturing fructose 1,6-diphosphate is based on the enzymatic phosphorylation of glucose with inorganic phosphate using permeabilized brewer's yeast cells. Our results demonstrate that a substantial improvement in the yield of bioconversion can be achieved using fed-batch-grown Saccharomyces cerevisiae cells. Under an appropriate glucose and phosphate to cell ratio the efficiency of bioconversion reaches 70% of the theoretical value.