Derek Cookson, P. Cheetham, E. Rathbone
1987
Citations
0
Influential Citations
8
Citations
Journal
Journal of Chromatography A
Abstract
Abstract The disaccharide 1-O-α- d -glucopyranosyl- d -fructose (trehalulose) was obtained in a 98% pure form, and in good yield by preparative-scale high-performance liquid chromatography (HPLC). Purification was from a complex mixture containing a trisaccharide, sucrose, isomaltulose, glucose and fructose, produced by immobilised microbial cells. Four reversed-phase preparative-scale HPLC columns were used in series with distilled water as the eluent. The structure of trehalulose was shown to be an equilibrium mixture of 1-O-α- d -glucopyranosyl-β- d -fructopyranose (66%) and 1-O-α- d -glucopyranosyl-β- d -fructofuranose (33%) by 13 C NMR spectroscopy and other methods. This work demonstrates how gram quantities of pure material can be conveniently obtained using preparative HPLC, even when the components in a mixture are difficult to resolve.