D. Perreaux, H. Maraite, J. Meyer
May 1, 1982
Citations
0
Influential Citations
25
Citations
Journal
Physiologial Plant Pathology
Abstract
Abstract A toxic substance was extracted by ethyl acetate from a broth culture of Xanthomonas campestris pv. manihotis acidified at pH 4 with 1 n HCl. When infiltrated into cassava leaves the crude extract produced blight symptoms similar to those induced by the bacteria during patho- The toxic substance was purified by gel filtration on a Sephadex G10 column (Pharmacia) and by thin layer chromatography on silica gel 60 (Merck), where it showed a RF of 0·6 using as migration system methanol : chloroform : n-hexane (3 : 2 : 1, v/v). By mass spectrometry, infrared spectroscopy, and C, H, N analyses the purified toxin was identified as 3(methylthio) propionic acid. This identification was confirmed by chemical synthesis. On detached cassava leaves, 3(methylthio) propionic acid gave a toxic effect up to a concentration of 9·9 m m corresponding to 24 μg of pure substance in a 20 μl drop of water.