K. Ou, M. Wilkins, Jun X. Yan
Feb 9, 1996
Citations
1
Influential Citations
55
Citations
Journal
Journal of Chromatography A
Abstract
Abstract An improved high-performance liquid chromatography method for the separation of amino acids derivatised with 9-fluorenylmethyl chloroformate (Fmoc) is described. This method, in conjunction with a multi-tasking program, not only allows high automatic througput but also offers baseline resolution of the common Fmoc-amino acids from a linear acetonitrile gradient, greater chromatographic reproducibility over the life of the column (800 runs) and easy column regeneration. The methods described are shown to be suitable for analysis of hydrolysates of proteins from two-dimensional gels for protein identification purposes, and will also be useful for routine quality control and screening of biological samples.