An improved method excluding hemoglobin interferences for lysosomal hydrolase assays using colorimetric synthetic substrates, 2-(N-hexadecanoylamino)-4-nitrophenol derivatives.

doi:10.1016/0003-2697(83)90627-9

Paper

DOI: 10.1016/0003-2697(83)90627-9

An improved method excluding hemoglobin interferences for lysosomal hydrolase assays using colorimetric synthetic substrates, 2-(N-hexadecanoylamino)-4-nitrophenol derivatives.

Published Apr 15, 1983 · T. Levade, R. Salvayre, J. Sicre

Analytical biochemistry

Q3 SJR score

Citations

Influential Citations

Full textSemantic Scholar

Abstract

Abstract hidden due to publisher request; this does not indicate any issues with the research. Click the full text link above to read the abstract and view the original source.

In Vitro Study

Study Snapshot

Selective extraction of 2-(N-hexadecanoylamino)-4-nitrophenol allows for lysosomal hydrolase assays without hemoglobin interference, enabling sphingomyelin phosphodiesterase, glucosylceramidase, and galact

PopulationOlder adults (50-71 years)

Sample size24

MethodsObservational

OutcomesBody Mass Index projections

ResultsSocial networks mitigate obesity in older groups.

Sign up to use Study Snapshot

Consensus is limited without an account. Create an account or sign in to get more searches and use the Study Snapshot.

Create an account

Full text analysis coming soon...

References

DOI: 10.1016/0009-8981(80)90143-6

The diagnosis of type A and type B Niemann Pick disease and detection of carriers using leukocytes and a chromogenic analogue of sphingomyelin.

Fresh leukocyte preparations can be used with confidence for diagnosis of Type A and Type B Niemann-Pick disease and detection of heterozygotes using HNP as substrate.

1980·16citations·A. E. Gal et al.·Clinica chimica acta; international journal of clinical chemistry

Clinica chimica acta; international journal of clinical chemistry

DOI: 10.1016/0009-8981(79)90195-5

Une micromethode simple pour la determination de l'activite sphingomyelinase

This rapid and sensitive micromethod for sphingomyelinase activity determination in leukocytes, fibroblasts, and hair roots uses the natural radioactive substrate and allows for determination in less than 0.5 g of fibroblast proteins.

1979·9citations·J. Mazière et al.·Clinica Chimica Acta

Clinica Chimica Acta

DOI: 10.1016/0009-8981(78)90427-8

Use of a chromogenic substrate for the diagnosis of Krabbe's disease, with special reference to its application in prenatal diagnosis.

The chromogenic substrate for Krabbe's disease diagnosis is less specific and less sensitive than natural substrates, making it insufficient for prenatal diagnosis.

1978·14citations·G. Besley et al.·Clinica chimica acta; international journal of clinical chemistry

Clinica chimica acta; international journal of clinical chemistry

DOI: 10.1016/0005-2760(77)90189-8

The isolation and characterization of sphingomyelinase from human placental tissue.

Human placental sphingomyelinase has a specificity for both hydrophilic phosphocholine and hydrophobic ceramide moieties, with potential interrelationships between heterogenous forms.

1977·55citations·P. Pentchev et al.·Biochimica et biophysica acta

Biochimica et biophysica acta

DOI: 10.1016/0009-8981(77)90401-6

A practical chromogenic procedure for the diagnosis of Krabbe's disease.

The synthesized analogue of galactocerebroside is a reliable chromogenic reagent for diagnosing Krabbe's disease and detecting heterozygous carriers of the Krabbe trait.

1977·39citations·A. E. Gal et al.·Clinica chimica acta; international journal of clinical chemistry

Clinica chimica acta; international journal of clinical chemistry

DOI: 10.1016/0026-0495(77)90080-4

Heritable catabolic and anabolic disorders of lipid metabolism.

Heritable disorders of lipid metabolism can now be diagnosed and prenatally detected, with enzyme replacement promising for patients with Fabry's and Gaucher's diseases.

1977·28citations·R. Brady·Metabolism: clinical and experimental

Metabolism: clinical and experimental

DOI: 10.1016/0003-2697(76)90527-3

A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.

This rapid and sensitive protein determination method uses Coomassie Brilliant Blue G-250 binding to protein, resulting in a shift in absorption maximum and good color stability for 1 hour.

1976·241168citations·M. M. Bradford·Analytical biochemistry

Analytical biochemistry

DOI: 10.1016/0009-3084(76)90015-3

Synthesis of 2-n-(hexadecanoyl)-amino-4-nitrophenyl phosphorylcholine-hydroxide, a chromogenic substrate for assaying sphingomyelinase activity.

This study synthesized 2-N-(hexadecanoyl)-amino-4-nitrophenyl phosphorylcholine-hydroxide, a chromogenic substrate for assaying sphingomyelinase activity, which resembles sphingomye

1976·22citations·A. E. Gal et al.·Chemistry and physics of lipids

Chemistry and physics of lipids

DOI: 10.1056/NEJM197509252931304

A practical chromogenic procedure for the detection of homozygotes and heterozygous carriers of Niemann-Pick disease.

The synthesized analogue of sphingomyelin is a reliable chromogenic reagent for diagnosing patients with Niemann-Pick disease and detecting heterozygous carriers of the disease trait.

1975·128citations·A. E. Gal et al.·The New England journal of medicine

The New England journal of medicine

Citations

DOI: 10.1016/j.nanoso.2024.101326

A comprehensive review on designing nanocomposite adsorbents for efficient removal of 4-nitrophenol from water

Nanocomposite adsorbents show potential for efficient removal of 4-nitrophenol from water, with potential for large-scale decontamination and environmental acceptance.

2024·15citations·N. El Messaoudi et al.·Nano-Structures & Nano-Objects

Nano-Structures & Nano-Objects

DOI: 10.1177/1087057104272546

A Novel High-Throughput Screening Format to Identify Inhibitors of Secreted Acid Sphingomyelinase

A novel high-throughput screening format identified novel compounds that inhibit secreted acid sphingomyelinase at pH 7.4, with both organic and bead-based extraction methods showing similar results and adaptability to 384-well format.

2005·14citations·R. Mintzer et al.·Journal of Biomolecular Screening

Journal of Biomolecular Screening

DOI: 10.1016/S0300-483X(00)00234-1

Hexachlorobenzene-induced alterations on neutral and acidic sphingomyelinases and serine palmitoyltransferase activities. A time course study in two strains of rats.

Hexachlorobenzene-induced decrease in hepatic sphingomyelin content may serve as a protective mechanism against liver injury, but strain-dependent.

2000·7citations·S. C. Billi de Catabbi et al.·Toxicology

Toxicology

DOI: 10.1111/J.1432-1033.1985.TB08940.X

Molecular forms of sphingomyelinase and non-specific phosphodiesterases in Epstein-Barr virus-transformed lymphoid cell lines from Niemann-Pick disease types A and B.

In lymphoid cell lines, sphingomyelinase and phosphodiesterases have separate genetic coding, and their activities are not reliable for diagnosing Niemann-Pick disease.

1985·5citations·T. Levade et al.·European journal of biochemistry

European journal of biochemistry

DOI: 10.1016/0005-2760(84)90337-0

Sphingomyelinase and nonspecific phosphodiesterase activities in Epstein-Barr virus-transformed lymphoid cell lines from Niemann-Pick disease A, B and C.

Deficient acid sphingomyelinase activity is found in Epstein-Barr virus-transformed lymphoid cell lines from Niemann-Pick disease types A and B, but not in those from type C and sea-blue histiocyte syndrome.

1984·16citations·T. Levade et al.·Biochimica et biophysica acta

Biochimica et biophysica acta