H. Guder, D. Heindl, H. Josel
2000
Citations
0
Influential Citations
1
Citations
Journal
Journal name not available for this finding
Abstract
One of the most sensitive and widespread colorimetric detection systems in matrix-based application formats is the redox couple 5-bromo-4-chloro-indolyl phosphate (BCIP)/nitroblue tetrazolium chloride (NBT). It is used for the detection and localisation of alkaline phosphatase activity especially in Western and Southern blots or in immunohisto/cytochemistry applications.Indolyl derivatives of type (I) are usually stable over a wide pH range, but after enzymatically catalyzed hydrolysis to the indolyl derivative (II) they rapidly form an indigoid dye (III) through atmospheric or chemical oxidation (see Figure 1).