B. D. Sippy, N. E. Engelbrecht, G. Hubbard
Sep 1, 2001
Citations
8
Influential Citations
254
Citations
Quality indicators
Journal
American journal of ophthalmology
Abstract
PURPOSE To evaluate potential toxic effects of indocyanine green dye on cultured human retinal pigment epithelial cells. METHODS Controlled laboratory experiment. Cultured human retinal pigment epithelial cells were exposed to balanced saline solution, balanced saline solution with endoillumination, indocyanine green or indocyanine green with endoillumination. Cells were evaluated by light microscopy, electron microscopy, and a mitochondrial dehydrogenase assay. RESULTS Retinal pigment epithelial cells exposed to indocyanine green showed no histologic or ultrastructural changes. Those exposed to indocyanine green alone or indocyanine green plus light demonstrated a significant decrease in mitochondrial enzyme activity (P = 0.0002 and 0.005, respectively). CONCLUSION Brief exposure of cultured human retinal pigment epithelial cells to indocyanine green results in decreased mitochondrial enzyme activity but does not appear to influence cellular morphology or ultrastructure.