D. Shafritz, F. Goodwin, H. Weissbach
Nov 1, 1969
Citations
0
Influential Citations
12
Citations
Quality indicators
Journal
Archives of biochemistry and biophysics
Abstract
Abstract In a previous study of in vitro brain ribosomal protein synthesis from this laboratory, solutions of oxidized catecholamines (aminochromes) were found to inhibit poly U dependent polyphenylalanine synthesis. Since aminochromes also inhibited phenylalanine polymerization in the well defined in vitro ribosomal system from E. coli , the latter was used to determine the effect of aminochromes on various intermediate partial reactions of protein synthesis. All of the partial reactions studied were inhibited by approximately 50% at aminochrome concentrations of 3–5 × 10 −4 m (10 times that required for equivalent inhibition of overall polymerization). In addition, using Sephadex G-50 and G-100 chromatography, it was determined that high molecular weight oxidized products from epinephrine (melanin precursors) are bound to ribosomes, and this may explain the observed inhibition.