M. B. Fairbanks, E. Kollar
Apr 1, 1974
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Influential Citations
21
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Quality indicators
Journal
Teratology
Abstract
This study examined the effects of hadacidin, an analogue of L-aspartic acid, on epithelial fusion, breakdown, and mesenchymal contact in explants of fetal mouse palatal shelves. Shelves from 14-day C57BL/6Js were explanted to agar-solidified Eagle's basal medium. Control palates grown in this medium mimicked normal closure; i.e., an epithelial seam formed, broke down, and mesenchymal contact occurred in vitro. Experimental palates were grown in this medium supplemented with 1 or 2 mg/ml hadacidin or with concentrations of L-aspartic acid equal to or in excess of that of hadacidin. Histological observations of explants exposed to 2 mg of hadacidin for 2 days indicated that cells of the marginal epithelia became adherent to form epithelial bands, but that subsequent epithelial breakdown and mesenchymal contact did not occur. The presence of mi to tic figures in the epithelial bands and in the mesenchyme of treated explants indicated that hadacidin inhibited breakdown, and, in addition, that it did not severely suppress or debilitate all cellular activity. This observation supports the fact that explants treated with hadacidin for 2 days, when moved to control medium for an additional 2 days, were able to undergo epithelial-band breakdown and mesenchymal penetration. Preliminary data from explants treated with hadacidin and L-aspartic acid indicated that competitive reversal by the amino acid did not occur consistently in vitro at concentrations of the amino acid 3 times that of hadacidin.