Madhukiran Parvathaneni, G. Battu, A. I. Gray
2014
Citations
1
Influential Citations
23
Citations
Quality indicators
Journal
Asian Pacific Journal of Tropical Disease
Abstract
Abstract Objective To investigate the in vitro and in vivo anticancer activities of hypophyllanthin and phyllanthin isolated from Phyllanthus amarus Schum & Thonn against breast cancer. Methods In vitro anticancer activity was evaluated against two cell lines (MCF-7 and MDA-MB-231) using MTT assay. In vivo anticancer activity was tested using Sprague-Dawley rats with N-methyl-N-nitrosourea induced mammary cancer. Results In vitro studies demonstrated a dose-dependent inhibitory effect on cell growth with IC 50 values of (35.18±1.48) μg/mL (hypophyllanthin) and (32.51±0.95) μg/mL (phyllanthin) for MCF-7; (38.74±1.24) (hypophyllanthin) and (32.2±1.17) (phyllanthin) for MDA-MB-231 breast cancer cell lines. Tumor weights per group at doses of 5 and 10 mg/kg/day for hypophyllanthin (12.82 and 12.06 g) and phyllanthin (11.95 and 8.87 g) treated groups were significantly ( P Conclusion Results of the present research work indicated that the isolated lignan compounds, hypophyllanthin and phyllanthin showed significant anticancer activities against breast cancer, in vitro and in vivo .