Isolation and Characterization of Desulfovibrio dechloracetivorans sp. nov., a Marine Dechlorinating Bacterium Growing by Coupling the Oxidation of Acetate to the Reductive Dechlorination of 2-Chlorophenol

doi:10.1128/AEM.66.6.2408-2413.2000

Paper

Isolation and Characterization of Desulfovibrio dechloracetivorans sp. nov., a Marine Dechlorinating Bacterium Growing by Coupling the Oxidation of Acetate to the Reductive Dechlorination of 2-Chlorophenol

Published Jun 1, 2000 · Baolin Sun, J. Cole, R. Sanford

Applied and Environmental Microbiology

Q1 SJR score

140

Citations

9

Influential Citations

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Abstract

ABSTRACT Strain SF3, a gram-negative, anaerobic, motile, short curved rod that grows by coupling the reductive dechlorination of 2-chlorophenol (2-CP) to the oxidation of acetate, was isolated from San Francisco Bay sediment. Strain SF3 grew at concentrations of NaCl ranging from 0.16 to 2.5%, but concentrations of KCl above 0.32% inhibited growth. The isolate used acetate, fumarate, lactate, propionate, pyruvate, alanine, and ethanol as electron donors for growth coupled to reductive dechlorination. Among the halogenated aromatic compounds tested, only the ortho position of chlorophenols was reductively dechlorinated, and additional chlorines at other positions blockedortho dechlorination. Sulfate, sulfite, thiosulfate, and nitrate were also used as electron acceptors for growth. The optimal temperature for growth was 30°C, and no growth or dechlorination activity was observed at 37°C. Growth by reductive dechlorination was revealed by a growth yield of about 1 g of protein per mol of 2-CP dechlorinated, and about 2.7 g of protein per mole of 2,6-dichlorophenol dechlorinated. The physiological features and 16S ribosomal DNA sequence suggest that the organism is a novel species of the genus Desulfovibrio and which we have designatedDesulfovibrio dechloracetivorans. The unusual physiological feature of this strain is that it uses acetate as an electron donor and carbon source for growth with 2-CP but not with sulfate.

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Study Snapshot

This novel marine dechlorinating bacteria uses acetate as an electron donor and carbon source for growth with 2-chlorophenol, but not with sulfate, making it a promising candidate for environmental applications.

PopulationOlder adults (50-71 years)

Sample size24

MethodsObservational

OutcomesBody Mass Index projections

ResultsSocial networks mitigate obesity in older groups.

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Paper

Isolation and Characterization of Desulfovibrio dechloracetivorans sp. nov., a Marine Dechlorinating Bacterium Growing by Coupling the Oxidation of Acetate to the Reductive Dechlorination of 2-Chlorophenol

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References

Fraction of Electrons Consumed in Electron Acceptor Reduction and Hydrogen Thresholds as Indicators of Halorespiratory Physiology

Chlororespiratory physiology can be reliably detected by measuring hydrogen consumption thresholds and tracking electrons transferred to chlorinated electron acceptors in both mixed and pure cultures.

Isolation from Estuarine Sediments of aDesulfovibrio Strain Which Can Grow on Lactate Coupled to the Reductive Dehalogenation of 2,4,6-Tribromophenol

Strain TBP-1, an anaerobic bacterium from estuarine sediments, can grow on lactate and dehalogenate 2,4,6-tribromophenol to phenol, with potential applications in bioremediation and bioremediation of toxic compounds.

Reductive dechlorination in the energy metabolism of anaerobic bacteria

Reductive dechlorination in anaerobic bacteria represents a new type of biochemical reaction, with some bacteria using it for energy conservation through 'dehalorespiration'.

Isolation and characterization of Desulfitobacterium frappieri sp. nov., an anaerobic bacterium which reductively dechlorinates pentachlorophenol to 3-chlorophenol.

Strain PCP-1T is a new species of anaerobic bacterium that dechlorinates pentachlorophenol to 3-chlorophenol, with a broader range of chlorophenol dechlorination than its parent, Desulfitobacterium dehalogenans JW

Characterization of Desulfitobacterium chlororespirans sp. nov., which grows by coupling the oxidation of lactate to the reductive dechlorination of 3-chloro-4-hydroxybenzoate

Strain Co23, a new species, grows by chlororespiration, removing ortho chlorines from polysubstituted phenols and reductive dechlorinating 3-chloro-4-hydroxybenzoate at temperatures up to 55 degrees C.

Citations

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Microorganisms like Clostridium and Desulfovibrio can enhance oil recovery by producing biosolvents, bioacids, biogases, and biosurfactants from simple nutritional compounds.

Anaerobic dihydrogen consumption of nutrient-limited aquifer sediment microbial communities examined by stable isotope analysis

Subsurface microbial communities can adapt to elevated H2 partial pressures, with methanogens and homoacetogens playing key roles in this process.

Biomolecular insights into the inhibition of heavy metals on reductive dechlorination of 2,4,6-trichlorophenol in Pseudomonas sp. CP-1.

Heavy metal exposure significantly inhibits organohalide respiration in Pseudomonas sp. strain CP-1, with Cu (II) showing the most serious inhibitory effects and As (V) showing the least inhibition.

Dehalococcoides mccartyi strain NIT01 grows more stably in vessels made of pure titanium rather than the stainless alloy SUS304

Dehalococcoides mccartyi strain NIT01 grows more stably and efficiently in titanium vessels, enabling faster dechlorination of trichloroethene in 12–28 days.