T. Noguchi, Y. Ueda, K. Hashimoto
1981
Citations
0
Influential Citations
9
Citations
Journal
Nippon Suisan Gakkaishi
Abstract
Gonyautoxin-1 (GTX1), one of the major components of paralytic shellfish poison, was isolated from the toxic digestive gland of scallop Patinopecten yessoensis by a method consisting of activated charcoal treatment and three types of column chromatography: on Bio-Gel P-2, Amberlite CG-50, and Bio-Rex 70. The scallop GTX1 thus obtained was homogeneous both in TLC and in cellulose acetate membrane electrophoresis. Elemental analysis and IR spectrometry indicated the presence of one mole of -OSO3- per mole of the toxin. This, together with the 1H-NMR and 13C-NMR spectra, suggested that the structure of GTX1 is 11-(OSO3) neosaxitoxin. The specific toxicity was determined to be 5, 000 MU/mg, which is comparable to that of saxitoxin, gonyautoxin-2, or tetrodotoxin.