Isolation and characterization of a Rhodococcus strain able to degrade 2-fluorophenol

doi:10.1007/s00253-011-3696-2

Paper

Isolation and characterization of a Rhodococcus strain able to degrade 2-fluorophenol

Published Nov 19, 2011 · A. Duque, S. A. Hasan, V. S. Bessa

Applied Microbiology and Biotechnology

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Abstract

A pure bacterial culture able to utilize 2-fluorophenol (2-FP) as sole carbon and energy source was isolated by selective enrichment from sediments collected from a contaminated site in Northern Portugal. 16S rRNA gene analysis showed that the organism (strain FP1) belongs to the genus Rhodococcus. When grown aerobically on 2-FP, growth kinetics of strain FP1 followed the Luong model. An inhibitory effect of increasing 2-FP concentrations was observed with no growth occurring at 2-FP levels higher than ca. 4 mM. Rhodococcus strain FP1 was able to degrade a range of other organofluorine compounds, including 2-fluorobenzoate, 3-fluorobenzoate, 4-fluorobenzoate, 3-fluorophenol, 4-fluorophenol, 3-fluorocatechol, and 4-fluorocatechol, as well as chlorinated compounds such as 2-chlorophenol and 4-chlorophenol. Experiments with cell-free extracts and partially purified enzymes indicated that the first step of 2-fluorophenol metabolism was conversion to 3-fluorocatechol, suggesting an unusual pathway for fluoroaromatic metabolism. To our knowledge, this is the first time that utilization of 2-FP as a growth substrate by a pure bacterial culture is reported.

Study Snapshot

Rhodococcus strain FP1 can utilize 2-fluorophenol as a sole carbon and energy source, degrading various organofluorine compounds and chlorinated compounds.

PopulationOlder adults (50-71 years)

Sample size24

MethodsObservational

OutcomesBody Mass Index projections

ResultsSocial networks mitigate obesity in older groups.

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Paper

Isolation and characterization of a Rhodococcus strain able to degrade 2-fluorophenol

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References

Isolation and Characterization of 4-tert-Butylphenol-Utilizing Sphingobium fuliginis Strains from Phragmites australis Rhizosphere Sediment

Sphingobium fuliginis strains from Phragmites australis rhizosphere sediment can degrade 4-tert-butylphenol as a sole carbon and energy source, potentially aiding in bioremediation of polluted environments.

Mineralization and transformation of monofluorophenols by Pseudonocardia benzenivorans

Pseudonocardia benzenivorans efficiently transforms monofluorophenols into 3- and 4-fluorocatechols, with ortho-cleavage pathway activity.

Biodegradation and biotransformation of organofluorine compounds

Microorganisms can degrade organofluorine compounds, potentially reducing environmental concerns.

Characterization of Chlorophenol 4-Monooxygenase (TftD) and NADH:FAD Oxidoreductase (TftC) of Burkholderia cepacia AC1100*

Burkholderia cepacia AC1100's TftD and TftC catalyze the complete degradation of 2,4,5-trichlorophenol, with TftC providing the required FADH2 and TftD using it to transform 2,4,5-TCP and 2,5-D

Analysis of Two Gene Clusters Involved in the Degradation of 4-Fluorophenol by Arthrobacter sp. Strain IF1

Arthrobacter sp. strain IF1 transforms 4-fluorophenol to hydroquinone by a two-component monooxygenase system, providing reduced flavin adenine dinucleotide and catalyzing para-hydroxylation of 4-substituted phenol

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