Isoprostane 8‐epi PGF2α, a product of oxidative stress, is synthesized in the bladder and causes detrusor smooth muscle contraction

doi:10.1002/(SICI)1520-6777(2000)19:1<43::AID-NAU6>3.0.CO;2-C

Paper

Isoprostane 8‐epi PGF2α, a product of oxidative stress, is synthesized in the bladder and causes detrusor smooth muscle contraction

Published 2000 · T. Tarcan, M. Siroky, R. Krane

Neurourology and Urodynamics

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43

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0

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Abstract

Isoprostane 8‐epi PGF2α is a product of oxidative stress that causes potent smooth muscle contraction. Its production increases in conditions associated with oxidative stress such as in diabetes, smoking, and aging. The aim was to study whether the urinary bladder synthesizes isoprostane 8‐epi PGF2α and releases to the urine and whether isoprostane 8‐epi PGF2α causes bladder smooth muscle contraction. Urine samples were obtained transurethrally from 12 male New Zealand white rabbits for measurement of isoprostane 8‐epi PGF2α levels. To examine whether bladder synthesizes isoprostane 8‐epi PGF2α, both ureters were ligated, then the bladder was washed 5 times by filling and emptying with normal saline. Bladder was refilled with normal saline, and at 5 minutes a bladder washout sample was taken. After this, the bladder was contracted by nerve stimulation periodically for 30 minutes, and then another washout sample was taken. Strips of bladder tissues were processed for study of isoprostane 8‐epi PGF2α production in tissue culture chambers and for isometric tension measurements in the organ bath. Enzyme immunoassay (EIA) revealed a remarkable amount of isoprostane 8‐epi PGF2α in the rabbit urine. EIA of washout samples showed that the bladder synthesizes isoprostane 8‐epi PGF2α and its production increases with nerve stimulation‐induced contractions. EIA of samples from the tissue culture media showed that bladder strips synthesize isoprostane 8‐epi PGF2α in vitro. Electrical field stimulation (EFS) significantly increased the synthesis and release of isoprostane 8‐epi PGF2α by the bladder strips. In the organ bath, isoprostane 8‐epi PGF2α caused concentration‐dependent contraction of bladder tissue. While the threshold contraction required smaller concentration of isoprostane 8‐epi PGF2α (3 nmol) than carbachol (10 nmol), the amplitude of contraction to carbachol was greater than isoprostane 8‐epi PGF2α. Our studies show that the rabbit bladder synthesizes isoprostane 8‐epi PGF2α and releases it to the urine. Production of isoprostane 8‐epi PGF2α in the bladder increases with nerve stimulation‐induced contraction. Exogenous isoprostane 8‐epi PGF2α causes significant bladder smooth muscle contraction. Our findings necessitate further studies to evaluate the possible role of oxidative stress and increased isoprostane 8‐epi PGF2α production in bladder dysfunction. Neurourol. Urodynam. 19:43–51, 2000. © 2000 Wiley‐Liss, Inc.

Non-RCT

Animal Study

Study Snapshot

The rabbit bladder synthesizes and releases isoprostane 8-epi PGF2, which causes significant bladder smooth muscle contraction, suggesting oxidative stress may play a role in bladder contraction.

PopulationOlder adults (50-71 years)

Sample size24

MethodsObservational

OutcomesBody Mass Index projections

ResultsSocial networks mitigate obesity in older groups.

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Paper

Isoprostane 8‐epi PGF2α, a product of oxidative stress, is synthesized in the bladder and causes detrusor smooth muscle contraction

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References

Effect of Repetitive Stimulation on the Contractile Response of Rabbit Urinary Bladder Subjected to in vitro Hypoxia or in vitro Ischemia Followed by Reoxygenation

Repetitive stimulation in rabbit urinary bladder tissue increases the rate of contractile dysfunction and is more sensitive to hypoxia and ischemia than nonrepetitive stimulation.

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Isoprostanes' effects on the canine colon involve both prostanoid TP and EP receptors.

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Epi-8-PGF2 alpha is a potent contractor of human peripheral lymphatics, potentially facilitating lymph transport in conditions of inflammation.

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Isoprostanes (8-iso-PGF2alpha and 8-iso-PGE2) are potent vasoconstrictors in isolated guinea pig hearts, with their action mediated by SQ 29548-responsive thromboxane receptors and their loss of

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Cavernosal ischemia due to arterial occlusive disease predicts abnormal veno-occlusive function and poor erection quality in an animal model of vasculogenic impotence.

Citations

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