K. Kanamori, B. Ross
Mar 1, 1999
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Magnetic Resonance in Medicine
Abstract
Three‐dimensional image‐selected in vivo spectroscopy (ISIS), combined with proton‐decoupled nuclear‐Overhauser‐enhanced 15N nuclear magnetic resonance (NMR), was used to localize [15N]metabolites, observed by a head coil, to the brain in rats. In spontaneously breathing anesthetized rats given intravenous [15N]ammonium acetate infusion, brain [5‐15N]glutamine was observed in the localized spectrum with a v1/2 of 5 Hz in 19–28 min at 4.7 T, while the signal from blood [15N]urea was eliminated by the localization process. In rats given [15N]leucine infusion, the peak representing predominantly (89%) brain [15N]glutamate was observed, with elimination of the signal from muscle [15N]alanine. In vivo peak areas of the brain [15N]metabolites in the localized spectra were proportional to their concentrations. The advantages and limitations of localization by ISIS using a volume coil with a homogeneous B1 field are compared with those of localization by a surface coil for in vivo 15N NMR study of neurotransmitters in the brain. Magn Reson Med 41:456–463, 1999. © 1999 Wiley‐Liss, Inc.