The phenolic monoterpenoid carvacrol inhibits the binding of nicotine to the housefly nicotinic acetylcholine receptor.

doi:10.1002/ps.3443

Paper

The phenolic monoterpenoid carvacrol inhibits the binding of nicotine to the housefly nicotinic acetylcholine receptor.

Published Jul 1, 2013 · F. Tong, A. Gross, M. Dolan

Pest management science

Q1 SJR score

84

Citations

3

Influential Citations

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Abstract

BACKGROUND The phenolic monoterpenoid carvacrol, which is found in many plant essential oils (thyme, oregano and Alaska yellow cedar), is highly active against pest arthropods, but its mechanisms of action are not fully understood. Here, carvacrol is shown to bind in a membrane preparation containing insect nicotinic acetylcholine receptors (nAChRs). [(14) C]-Nicotine binding assays with Musca domestica (housefly) nAChRs were used in this study to demonstrate carvacrol's binding to nAChRs, thereby acting as a modulator of the receptors. RESULTS Carvacrol showed a concentration-dependent inhibition of [(14) C]-nicotine binding in a membrane preparation of housefly heads containing nAChRs, with IC50 = 1.4 μM, in a non-competitive pattern. Binding studies with neonicotinoid insecticides revealed that imidacloprid and thiamethoxam did not inhibit the binding of [(14) C]-nicotine, while dinotefuran, from the guanidine subclass of neonicotinoids, inhibited nicotine binding like carvacrol. CONCLUSIONS Carvacrol binds to housefly nAChRs at a binding site distinct from nicotine and acetylcholine, and the nAChRs are a possible target of carvacrol for its insecticidal activity.

In Vitro Study

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Study Snapshot

Carvacrol, found in plant essential oils, inhibits nicotine binding to housefly nicotinic acetylcholine receptors, potentially explaining its insecticidal activity.

PopulationOlder adults (50-71 years)

Sample size24

MethodsObservational

OutcomesBody Mass Index projections

ResultsSocial networks mitigate obesity in older groups.

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Paper

The phenolic monoterpenoid carvacrol inhibits the binding of nicotine to the housefly nicotinic acetylcholine receptor.

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References

Acetylcholinesterase inhibition by nootkatone and carvacrol in arthropods

Nootkatone and carvacrol's insecticidal activity is likely not primarily based on acetylcholinesterase inhibition, as their primary mode of action is likely through other mechanisms.

Thiamethoxam, a poor agonist of nicotinic acetylcholine receptors expressed on isolated cell bodies, acts as a full agonist at cockroach cercal afferent/giant interneuron synapses

Thiamethoxam acts as a full agonist of nicotinic acetylcholine receptors at cockroach cercal afferent/giant interneuron synapses, suggesting it may bind to a'mixed nicotinic

Effects of monoterpenoid insecticides on [3H]-TBOB binding in house fly GABA receptor and 36Cl− uptake in American cockroach ventral nerve cord

Carvacrol, pulegone, and thymol are positive allosteric modulators at insect GABA receptors, while -terpineol and linalool show little or no effect.

Nematicidal activity of two monoterpenoids and SER-2 tyramine receptor of Caenorhabditis elegans.

Thymol and carvacrol show nematicidal activity in nematodes, potentially mediated through the SER-2 tyramine receptor, offering a potential screening platform for developing new antihelmintic compounds.

Ability of Two Natural Products, Nootkatone and Carvacrol, to Suppress Ixodes scapularis and Amblyomma americanum (Acari: Ixodidae) in a Lyme Disease Endemic Area of New Jersey

Nootkatone and carvacrol effectively suppress Ixodes scapularis and Amblyomma americanum tick populations at low concentrations, potentially offering a future alternative to conventional synthetic acaricides.

Citations

The impact of carvacrol on the larval gut bacterial structure and function of Lymantria dispar

Carvacrol treatment significantly alters gut bacteria in Lymantria dispar larvae, shifting dominant bacteria from Proteobacteria to Firmicutes, and enhancing probiotic Lactobacillus abundance, potentially reducing insecticidal activity.

Chemical Composition, Antioxidant and Anti-Enzymatic Activities, and In Vitro Insecticidal Potential of Origanum compactum (Benth.) Essential Oils

Origanum compactum essential oils show strong inhibitory effects against enzymes AChE, tyrosinase, and -glucosidase, with potential medicinal applications and pest control properties.

Curcumin and carvacrol co-loaded zein nanoparticles: comprehensive preparation and assessment of biological activities in pest control

Curcumin and carvacrol nanoparticles encapsulated in zein show promising biological activity against pests like soybean pests, mites, and caterpillars without causing phytotoxicity.

A Decade Review of Analysis of Essential Oils from Genus Artocarpus: its Phytochemistry and Pharmacology

Essential oils from Artocarpus species show potent antioxidant capacity, potentially aiding in the preservation of good health and treating various chronic disorders.

In vitro acaricidal activity of essential oils and their binary mixtures against ixodes scapularis (Acari: Ixodidae).

Essential oils from lemongrass, geranium, savory thyme, and white thyme show strong acaricidal activity against ticks, with potential for eco-friendly tick control products.

The Potential of Plant-Based Biorational Products for the Drosophila suzukii Control: Current Status, Opportunities, and Limitations.

Plant-based biorational insecticides show potential in controlling Drosophila suzukii, but more research is needed to address potential negative effects on beneficial organisms and assess their efficacy in field conditions.

The effects of carvacrol on development and gene expression profiles in Spodoptera frugiperda.

Carvacrol inhibits Spodoptera frugiperda larval growth and mortality by affecting food digestion and interacting with various insecticidal targets.