B. Brian, J. Boltralik, L. Thom
Apr 1, 1974
Citations
0
Influential Citations
5
Citations
Journal
Journal of pharmaceutical sciences
Abstract
A rapid, efficient, and sensitive method was developed for extraction and subsequent quantitation of aceclidine (3-acetoxyquinuclidine) hydrochloride and 3-quinuclidinol hydrochloride from biological fluid. After adjustment of pH and salt concentration, chloroform extracts of serum, urine, or aqueous fluid could be quantitated by GLC without derivative formation. The analytical procedure was used to determine the corneal absorption of aceclidine in the rabbit eye. Acelidine, a drug used topically in glaucoma treatment, was found to enter the anterior chamber of the eye by penetration exclusively through the cornea. In experiments on conjunctival absorption, the amount found in the anterior chamber was less than 50 ng. The corneal absorption experiments gave cumulated absorption of from 1 to 8.5 μg in 30 min.