Optimization of 1(3)-Palmitoyl-2-Oleoyl-3(1)-Stearoyl Glycerol Produced via Lipase-catalyzed Esterification Using the Response Surface Methodology

doi:10.11002/KJFP.2011.18.5.721

Paper

Optimization of 1(3)-Palmitoyl-2-Oleoyl-3(1)-Stearoyl Glycerol Produced via Lipase-catalyzed Esterification Using the Response Surface Methodology

Published Oct 30, 2011 · Y. Hwang, Jung‐Ah Shin, Jeung-Hee Lee

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Abstract

1(3)-palmitoyl-2-oleoyl-3(1)-stearoyl-(POS)-glycerol-enriched reaction products were synthesized from camellia oil, palmitic ethyl ester, and stearic ethyl ester via lipase-catalyzed interesterification. Response surface methodology (RSM) was employed to optimize the production of the POS-enriched reaction product (Y1, %) and the stearicand palmitic-acid contents at the sn-2 position due to acyl migration (Y2, %). The reaction factors were the enzyme amount (X1, 2-6%), reaction time (X2, 60-360 min), and substrate molar ratio of camellia oil to palmitic ethyl ester and stearic ethyl ester (X3, 1-3 mol). The predictive models for Y1 and Y2 were adequate and reproducible as no lack of fit was signified (0.128 and 0.237) and as there were satisfactory levels of R2 (0.968 and 0.990, respectively). The optimal conditions for the reaction product for maximizing Y1 while minimizing Y2 were predicted at the reaction combination of 5.86% enzyme amount, 60 min reaction time, and 1:3 substrate molar ratio (3 moles of palmitic ethyl ester and 3 moles of stearic ethyl ester). Actual reaction was performed under the same conditions as above, and the resulting product contained 20.19% TAG-P/O/S and 12.71% saturated fatty acids at the sn-2 position.

Study Snapshot

The optimal conditions for producing 1(3)-palmitoyl-2-oleoyl-3(1)-stearoyl glycerol from camellia oil, palmitic ethyl ester, and stearic ethyl ester using response surface methodology are 5.86% enzyme

PopulationOlder adults (50-71 years)

Sample size24

MethodsObservational

OutcomesBody Mass Index projections

ResultsSocial networks mitigate obesity in older groups.

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Paper

Optimization of 1(3)-Palmitoyl-2-Oleoyl-3(1)-Stearoyl Glycerol Produced via Lipase-catalyzed Esterification Using the Response Surface Methodology

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References

Cocoa butter equivalent from enzymatic interesterification of tea seed oil and fatty acid methyl esters

Cocoa butter equivalent (CBE) can be prepared by interesterifying tea seed oil, methyl palmitate, and methyl stearate with lipase, resulting in a product with similar melting range and dilatation-temperature curves to cocoa butter.

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Using response surface methodology, we optimized lipase-catalyzed acidolysis of sesame oil and caprylic acid for structured lipids, finding that acyl migration should be kept below 55 mol% to maintain quality and yield.

Diacylglycerol synthesis by enzymatic glycerolysis: Screening of commercially available lipases

Novozym 435 and Lipase PS-D show the best diacylglycerol synthesis in rapeseed oil glycerolysis, with all lipases showing potential for enzymatic glycerolysis with glycerol absorbed on silica

Cluster analysis for the systematic grouping of genuine cocoa butter and cocoa butter equivalent samples based on triglyceride patterns.

Cluster analysis of five main triglycerides can effectively group cocoa butters and cocoa butter equivalents, aiding in assessing their purity and origin.

Cocoa butter equivalent through enzymic interesterification of palm oil midfraction

Enzymic interesterification of palm oil midfraction with stearic acid in a solvent-free system can produce a cocoa butter equivalent with high specific productivity and mass productivity.

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