I. Enemali, M. Garba, O. Obodozie
Jun 18, 2007
Citations
1
Influential Citations
5
Citations
Journal
African Journal of Biotechnology
Abstract
Samples were extracted with dichloromethane and the organic layer evaporated to dryness. The residue was dissolved in methanol, and 25 il aliquot injected onto the column. Tolbutamide was used as the internal standard for chlorpropamide. The UV detector response was linear over the range 0 – 300 ig/ml, with a correlation coefficient of 0.999 and detection limit of 1.30 ng/ml. Within day and betweenday assay variations was generally observed. The utility of the method was demonstrated by determine chlorpropamide in samples from human volunteers following a single oral dose of 250 mg in drug interaction studies. The procedure is simple and fast, requiring small volumes of plasma.