Photosensitization by 3,3'-dihexyloxacarbocyanine iodide: specific disruption of microtubules and inactivation of organelle motility.

Paper

Photosensitization by 3,3'-dihexyloxacarbocyanine iodide: specific disruption of microtubules and inactivation of organelle motility.

Published May 15, 1995 · Christopher Lee, Samuel S. Wu, Lan Bo Chen

Cancer research

Q1 SJR score

49

Citations

4

Influential Citations

Semantic Scholar

Abstract

Photodynamic therapy is a useful new direction for cancer treatment. However, relatively little is currently known about the cellular targets and processes underlying the efficacy of these therapies. In this study, we report evidence of specific photosensitization of a novel intracellular target, cytoskeletal microtubules, that has great importance for cancer treatment. Photosensitization destroys microtubules, halts intracellular organelle motility processes, and leads to rapid cell death. We have examined the cell biological effects of photosensitization with the carbocyanine dye 3,3'-dihexyloxacarbocyanine iodide, which concentrates in mitochondria and the endoplasmic reticulum. Exposure of stained CV-1 kidney epithelial cells to as little as 30-120 s standard fluorescence excitation light caused disruption of the interphase microtubule network and complete inhibition of motility of the endoplasmic reticulum and all phase-contrast visible organelles, as specific effects of dye photoexcitation. Photoexcitation of rhodamine 123 or Hoechst produced neither of these effects. Furthermore, 3,3'-dihexyloxacarbocyanine iodide-mediated photodamage was specific to microtubules; other elements of the cytoskeleton, including vimentin intermediate filaments and actin stress fibers, were unaffected. We have reproduced the photoinactivation of microtubules in vitro with purified microtubule proteins.

In Vitro Study

Study Snapshot

The 3,3'-dihexyloxacarbocyanine iodide dye specifically disrupts microtubules and inactivates organelle motility, potentially benefiting cancer treatment by targeting specific intracellular targets.

PopulationOlder adults (50-71 years)

Sample size24

MethodsObservational

OutcomesBody Mass Index projections

ResultsSocial networks mitigate obesity in older groups.

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Paper

Photosensitization by 3,3'-dihexyloxacarbocyanine iodide: specific disruption of microtubules and inactivation of organelle motility.

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References

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Photodynamic therapy-induced oxidative stress leads to DNA damage, inhibition of poly(ADP-ribosyl)ation, and DNA fragmentation in murine L929 fibroblasts.

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Photodynamically induced reproductive cell death pathways are different for different cell types, with inhibition of Na+K+-ATPase activity, amino-acid transport inhibition, and mitochondrial dysfunction being crucial.

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Microspectrofluorometry can effectively study photosensitizer localization in single living cells, enabling more precise targeting and monitoring of photochemotherapeutic reactions.

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Photodynamic therapy (PDT) is a promising oncologic treatment for various malignancies, with potential as an intraoperative adjuvant or primary treatment for superficial malignancies.

Taxol: the first of the taxanes, an important new class of antitumor agents.

Taxol shows significant antineoplastic activity in advanced ovarian cancer and may be the "anticancer drug of the 1990s," with potential in other advanced cancers.

Citations

Photodynamic sensitizers modulate cytoskeleton structural dynamics in neuronal cells

Photodynamic therapy (PDT) may have potential against Alzheimer's disease and other neurodegenerative disorders by modulating cytoskeleton proteins and inducing apoptosis, DNA damage, and inflammation.

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TMPyP-PDT induces mitotic catastrophe and microtubule disorganization in HeLa and G361 cells, leading to cell death through mitotic catastrophe mechanisms.

The role of cytoskeleton and adhesion proteins in the resistance to photodynamic therapy. Possible therapeutic interventions

Photodynamic Therapy (PDT) alters tumor cells' cytoskeleton and adhesion proteins, potentially contributing to resistance and metastasis, and targeting these molecules may be a potential therapeutic strategy.

Zebrafish xenotransplantation model for cancer stem-like cell study and high-throughput screening of inhibitors

Zebrafish xenotransplantation models effectively study cancer stem-like cell mechanisms and enable high-throughput screening of anti-CSC inhibitors.

Synthesis and Characterization of Near‐Infrared Absorbing Water Soluble Squaraines and Study of their Photodynamic Effects in DLA Live Cells

SQDI and SQMI show higher cytotoxicity in DLA live cells, while ASQI shows lower cytotoxicity and stable photodynamic effects.

Never say dye

DiOC6 is a versatile fluorescent dye with multiple applications in cell biology, offering high-specificity labeling of cell structures, organelles, and metabolites.

Uptake and localization mechanisms of fluorescent and colored lipid probes. 1. Physicochemistry of probe uptake and localization, and the use of QSAR models for selectivity prediction

Fluorescent and colored lipid probes accurately target lipids and membranes, but face biological and methodological limitations due to biomembrane composition, cell line effects, and toxicity.

Photodynamic therapy with hexyl aminolevulinate induces carbonylation, posttranslational modifications and changed expression of proteins in cell survival and cell death pathways

Photodynamic therapy with hexyl aminolevulinate (HAL) induces complex cellular responses, affecting proteins involved in cell survival and cell death pathways, potentially improving clinical efficacy.

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Photodynamic therapy resistance may involve altered drug uptake, efflux rates, and intracellular trafficking, with stress response genes, antioxidant enzymes, and heat shock proteins playing key roles.