Piperine, an alkaloid from black pepper, inhibits growth of human colon cancer cells via G1 arrest and apoptosis triggered by endoplasmic reticulum stress

doi:10.1002/mc.22176

Paper

Piperine, an alkaloid from black pepper, inhibits growth of human colon cancer cells via G1 arrest and apoptosis triggered by endoplasmic reticulum stress

Published Oct 1, 2015 · Paul B Yaffe, Melanie R. Power Coombs, Carolyn D. Doucette

Molecular Carcinogenesis

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149

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3

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Abstract

Piperine, a piperidine alkaloid present in black pepper, inhibits the growth of cancer cells, although the mechanism of action is not well understood. In this study, we show that piperine (75–150 µM) inhibited the growth of several colon cancer cell lines but had little effect on the growth of normal fibroblasts and epithelial cells. Piperine inhibited HT‐29 colon carcinoma cell proliferation by causing G1 phase cell cycle arrest that was associated with decreased expression of cyclins D1 and D3 and their activating partner cyclin‐dependent kinases 4 and 6, as well as reduced phosphorylation of the retinoblastoma protein and up‐regulation of p21/WAF1 and p27/KIP1 expression. In addition, piperine caused hydroxyl radical production and apoptosis that was partially dependent on the production of reactive oxygen species. Piperine‐treated HT‐29 cells showed loss of mitochondrial membrane integrity and cleavage of poly (ADP‐ribose) polymerase‐1, as well as caspase activation and reduced apoptosis in the presence of the pan‐caspase inhibitor zVAD‐FMK. Increased expression of the endoplasmic reticulum stress‐associated proteins inositol‐requiring 1α protein, C/EBP homologous protein, and binding immunoglobulin protein, and activation of c‐Jun N‐terminal kinase and p38 mitogen‐activated protein kinase, as well as decreased phosphorylation of Akt and reduced survivin expression were also observed in piperine‐treated HT‐29 cells. Furthermore, piperine inhibited colony formation by HT‐29 cells, as well as the growth of HT‐29 spheroids. Cell cycle arrest and endoplasmic reticulum stress‐associated apoptosis following piperine treatment of HT‐29 cells provides the first evidence that piperine may be useful in the treatment of colon cancer. © 2014 Wiley Periodicals, Inc.

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Piperine, an alkaloid found in black pepper, inhibits colon cancer cell growth by causing G1 arrest and apoptosis triggered by endoplasmic reticulum stress, suggesting potential for colon cancer treatment.

PopulationOlder adults (50-71 years)

Sample size24

MethodsObservational

OutcomesBody Mass Index projections

ResultsSocial networks mitigate obesity in older groups.

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Paper

Piperine, an alkaloid from black pepper, inhibits growth of human colon cancer cells via G1 arrest and apoptosis triggered by endoplasmic reticulum stress

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References

Antitumor efficacy of piperine in the treatment of human HER2-overexpressing breast cancer cells.

Piperine, a component of black pepper, shows potential as a treatment for HER2-overexpressing breast cancer by inhibiting tumor growth, inducing apoptosis, and enhancing sensitivity to paclitaxel.

Piperine inhibits IL-β induced expression of inflammatory mediators in human osteoarthritis chondrocyte.

Piperine, the active component in black pepper, effectively inhibits IL-1-induced over-expression of inflammatory mediators in human osteoarthritis chondrocytes, suggesting it may be a potential agent in the treatment of OA.

Piper nigrum and Piperine: An Update

Black pepper and piperine have significant medicinal properties, including chemoprevention, detoxification, and enhanced absorption and bioavailability of herbal and conventional drugs.

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Piperine, a component of black pepper, shows potential anti-tumor effects by inhibiting prostate cancer cell proliferation, inducing apoptosis, disrupting androgen receptor expression, and reducing prostate specific antigen levels.

Identifying Molecular Targets of Lifestyle Modifications in Colon Cancer Prevention

Lifestyle interventions targeting molecular targets like dietary carcinogens, dietary deficiencies, and chronic inflammation could potentially reduce colorectal cancer risk and improve cancer prevention strategies.

Citations

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