B. Gourmel, J. Fiet, R. Collins
Sep 10, 1981
Citations
0
Influential Citations
4
Citations
Journal
Clinica chimica acta; international journal of clinical chemistry
Abstract
Acebutolol or DL1-(2-acetyl-4 butyramidophenoxy)-2-hydroxy-3-isopropylaminopropane metabolizes into three metabolites. Metabolite I i.e. diacetolol (DL-l(2-acetyl-4 acetamidophenoxy)-2-hydroxy-3 isopropyl-aminopropane) is the most important pharmacologically, since it has anti-arrhythmic and P-blocking properties resembling those of acebutolol [ 11. Chromatographic methods such as combined gas chromatography [2] and high pressure liquid chromatography (HPLC) [3,4] have allowed specific measurement of both acebutolol and diacetolol, but require prior extraction, and although some of them are sensitive [4], their sensitivity is lower than that of radioimmunoassay. This is why, on the basis of our previous method of acebutolol measurement [5], we have developed a fast radioimmunoassay (RIA) for diacetolol determination without extraction. This method is more suitable for pharmacological tests than its predecessors.