Zhang Ke-y
2014
Citations
0
Influential Citations
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Citations
Quality indicators
Journal
Chinese journal of veterinary science
Abstract
3-methylquinoxaline-2-carboxylate is one of the major metabolites quinocetone and olaquindox.For evaluation the safety of 3-methylquinoxaline-2-carboxylate,we prepared 3-methylquinoxaline-2-carboxylate by Beirut reaction,reduction reaction and decarboxylation in vitro,identified its physical and chemical characteristics,and investigated its antibacterial activity,studied the cell growth inhibition rates by MTT assay,evaluated the DNA damages by single cell gel electrophoresis,examined the changes of cell cycle with flow cytometry,respectively.The results showed that 3-methylquinoxaline-2-carboxylate was successfully synthesized and has not antibacterial activity.The inhibition rates of 3-methylquinoxaline-2-carboxylate within the detection dose range were less than 30%displayed the weak cells growth inhibitions effects on various cell lines.We found the DNA damage induced by 3-methylquinoxaline-2-carboxylate at higher doses,expressed as tail length and tail DNA was significantly increased;3-methylquinoxaline-2-carboxylate also changed the cell cycle on Chang liver cells with the S phase arrest.The results suggest 3-methylquinoxaline-2-carboxylate has a certain degree cytotoxicity on various cell lines.