J. Heinonen, Henna Niskakoski, Baoru Yang
Sep 1, 2016
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0
Influential Citations
3
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Journal
Journal of Chemical Technology & Biotechnology
Abstract
BACKGROUND Enzymatic synthesis and chromatographic purification of ethyl β-D-glucopyranoside was investigated. Ethyl β-D-glucopyranoside was produced from glucose and ethanol with β-glucosidase enzyme (EC 3.2.1.21). Cation exchange resins in Ca2+ and Na+ forms were used for the purification of ethyl-β-D-glucopyranoside. RESULTS Greater than 60% conversion was obtained in the synthesis. The reaction which follows the double displacement mechanism was successfully modelled with a simple kinetic model. Good separation of ethyl-β-D-glucopyranoside and glucose was obtained with weak acid cation exchange resins in Na+ form. 5.5 wt% crosslinked resin performed slightly better than 8 wt% crosslinked resin. The separation is based on hydrophobic interactions. Presence of ethanol disturbs the separation and should be removed before the chromatographic step. Scale-up of the purification step (bed volume: 0.11 L to 1.9 L) was successfully carried out to produce ethyl-β-D-glucopyranoside with 99% purity. Performance of the purification process was evaluated with numerical simulations. With respect to ethyl-β-D-glucopyranoside, the separation performance was found to go through a shallow maximum with increasing column loading and flow rate. CONCLUSION An efficient chromatographic purification method was developed for enzymatically produced ethyl-β-D-glucopyranoside. This method could be utilized also in the purification of other enzymatically produced glucopyranosides. © 2015 Society of Chemical Industry