T. Hamid, Azzmer Azzar Abdul Hamid, A. H. Zulkifly
Jan 24, 2011
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Journal
African Journal of Biotechnology
Abstract
Halogenated compounds are widely used in agriculture and industries and have been associated with environmental pollution. Degradation of 3-chloropropionate (3CP) by microorganism has been established and this enzyme could only remove halogen atom at the â- position of 3-carbon alkanoic acids. Pseudomonas sp . B6P was originally isolated from paddy field which was able to degrade 3CP therefore, suggesting it may have some desirable properties. The enzyme was purified from cell-free extracts having a monomer of 56,000 Da. It was found to be stable between pH 5 to 8 and its optimal pH was 8. Its activity was not affected by metal ions such as Mn 2+ , Fe 3+ and Mg 2+ , but was inhibited by Hg 2+ and Ag 2+ . The enzyme is specific for 3CP, and the Km value (0.20 mM ± 0.05). Key words : Biodegradation, 3-chloropropionic acid, dehalogenase, bioremediation, haloalkanoic acid, Pseudomonas sp.B6P.