Paper
iTRAQ‐Based Quantitative Proteomic Analysis of the Anticancer Mechanism of a Novel Ent‐kaurane Diterpenoid from RubuscorchorifoliusL.f
Published Apr 1, 2016 · Xue-xiang Chen, Zili Gao, Yangwen Ou
The FASEB Journal
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Abstract
Our previous study has identified (16R)‐16,17‐dihydroxy‐ent‐ kaur‐2‐one (a novel ent‐kaurane diterpenoid) from Rubuscorchorifolius L.f.as a potential anti‐cancer agent. Herein, we determined the mechanism of action of the diterpenoid in inhibiting the growth of human colon cancer cells. The results from flow cytometry analysis showed that the diterpenoid induced extensive apoptosis and cell cycle arrest in human colon cancer cells. iTRAQ‐Based Quantitative Proteomic analysis revealed that treatment with the diterpenoid led to upregulation of 67 proteins and down regulation of 53 proteins. These proteins play important roles in multiple cellular processes such as enzyme activity adjusting, electron transferring, anti‐oxidation and protein transcription. These proteins also are critical in path ways related to carcinogenesis such as oxidative phosphorylation pathway, cell cycle regulation, and p53 pathway. Treatment with the diterpenoid modulated the expression levels of APC, DCC, TGFβRII, Smad2m, Bax, p53 and 14‐3‐3σ, which may contribute to its inhibitory effects on colon cancer cells. Overall, our work produce dinitial mechanistic information on the action of (16R)‐16,17‐dihydroxy‐ent‐ kaur‐2‐one in inhibiting the growth of colon cancer cells, which provided important scientific basis for further investigation.
The novel ent-kaurane diterpenoid from Rubuscorchorifolius L.f. inhibits colon cancer cell growth by inducing apoptosis and cell cycle arrest, with potential therapeutic implications for treating various cancers.
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