H. Sinn, H. Schrenk, E. Friedrich
Apr 1, 1988
Citations
1
Influential Citations
78
Citations
Quality indicators
Journal
Analytical biochemistry
Abstract
In an attempt to improve conditions for radioiodination of sensitive proteins we used N-bromosuccinimide as a mild oxidizing agent. Under gentle conditions we increased the average labeling efficiency of a wide variety of proteins to above 97%. There was no loss of binding activity of low density lipoprotein particles, which are most sensitive to oxidation. Depending on high labeling efficiency, our method reduces preparation time as well as radioactive waste, costs, and irradiation exposure to personnel.