Initial Reactions in the Biodegradation of 1-Chloro-4-Nitrobenzene by a Newly Isolated Bacterium, Strain LW1

doi:10.1128/AEM.65.4.1405-1412.1999

Paper

Initial Reactions in the Biodegradation of 1-Chloro-4-Nitrobenzene by a Newly Isolated Bacterium, Strain LW1

Published Apr 1, 1999 · E. Katsivela, V. Wray, D. Pieper

Applied and Environmental Microbiology

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78

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10

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Abstract

ABSTRACT Bacterial strain LW1, which belongs to the familyComamonadaceae, utilizes 1-chloro-4-nitrobenzene (1C4NB) as a sole source of carbon, nitrogen, and energy. Suspensions of 1C4NB-grown cells removed 1C4NB from culture fluids, and there was a concomitant release of ammonia and chloride. Under anaerobic conditions LW1 transformed 1C4NB into a product which was identified as 2-amino-5-chlorophenol by 1H and 13C nuclear magnetic resonance (NMR) spectroscopy and mass spectrometry. This transformation indicated that there was partial reduction of the nitro group to the hydroxylamino substituent, followed by Bamberger rearrangement. In the presence of oxygen but in the absence of NAD, fast transformation of 2-amino-5-chlorophenol into a transiently stable yellow product was observed with resting cells and cell extracts. This compound exhibited an absorption maximum at 395 nm and was further converted to a dead-end product with maxima at 226 and 272 nm. The compound formed was subsequently identified by 1H and 13C NMR spectroscopy and mass spectrometry as 5-chloropicolinic acid. In contrast, when NAD was added in the presence of oxygen, only minor amounts of 5-chloropicolinic acid were formed, and a new product, which exhibited an absorption maximum at 306 nm, accumulated.

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Study Snapshot

Bacterial strain LW1 efficiently degrades 1-chloro-4-nitrobenzene into 2-amino-5-chlorophenol and 5-chloropicolinic acid under anaerobic conditions, with potential applications in biofuel production.

PopulationOlder adults (50-71 years)

Sample size24

MethodsObservational

OutcomesBody Mass Index projections

ResultsSocial networks mitigate obesity in older groups.

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Paper

Initial Reactions in the Biodegradation of 1-Chloro-4-Nitrobenzene by a Newly Isolated Bacterium, Strain LW1

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References

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Microbial systems can biodegrade nitroaromatic compounds, potentially benefiting environmental cleanup and synthesis of valuable organic molecules.

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The novel 2-aminomuconate deaminase in Pseudomonas pseudoalcaligenes JS45 effectively degrades nitrobenzene by directly converting 2-aminomuconate to 4-oxalocrotonate, with ammonia as the nitrogen source for

Citations

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Bacterial consortia can effectively biodegrade insensitive munitions compounds and co-occurring nitroaromatics in waste streams, with complete biodegradation achieved by switching redox conditions.

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The strains identified in this study are resistant to high hexachlorobenzene concentrations and belong to the species Comamonas testosteron UCM B-400 and UCM B-401, which are motile gram-negative rods and aerobic chemoorganotrophic.

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A Nag-like dioxygenase initiates 3,4-dichloronitrobenzene degradation via 4,5-dichlorocatechol in Diaphorobacter sp. strain JS3050.

Diaphorobacter sp. strain JS3050 efficiently degrades 3,4-dichloronitrobenzene into 4,5-dichlorocatechol, avoiding toxic intermediate misrouting and enhancing understanding of microbial catabolic diversity in response to environmental pollutants.

Bioaugmentation ofp‐chloronitrobenzene in bioelectrochemical systems withPseudomonas fluorescens

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Engineering the bacterium Comamonas testosteroni CNB-1: Plasmid curing and genetic manipulation

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