G. Clement, G. S. Cashell
Apr 1, 1972
Citations
0
Influential Citations
2
Citations
Journal
Biochemical and biophysical research communications
Abstract
Abstract The kinetics of the rennin catalyzed hydrolysis of Z-His-Phe-Trp-OEt ∗ to produce Z-His-Phe and Trp-OEt in the acid pH range have been measured. The pH-E o k cat K m curve is bell shaped with pKa's 2.9 and 5.4. The decrease in the k cat K m values at pH's m with decreasing pH. Rennin was found to be unstable below pH 2 with good stability exhibited between pH 2 and 6. A method for chromatographing rennin and determining its milk clotting activity is presented.