J. Hobbie, C. C. Crawford
Jul 1, 1969
Citations
7
Influential Citations
380
Citations
Quality indicators
Journal
Limnology and Oceanography
Abstract
The uptake of 14C-labeled organic compounds has been used by many workers to study heterotrophic microorganisms in natural waters. However, if flux rates of organic compounds are to be measured, the loss of 14CO2 during incubation becomes an important source of error. A method is proposed in which the experiment is run in a closed system and the 14CO2 collected after killing and acidification. Phenethylamine on chromatographic paper is the absorbing agent and the paper is counted by liquid scintillation. Studies of 19 compounds from pond water showed that 60% (aspartic acid) to 8% (arginine) of the labeled material entering the microorganisms was respired.