Role of alpha-tocopherol in counteracting DNA damage induced by Ochratoxin A in primary porcine fibroblasts

doi:10.4081/ijas.2009.s2.301

Paper

Role of alpha-tocopherol in counteracting DNA damage induced by Ochratoxin A in primary porcine fibroblasts

Published Jan 1, 2009 · E. Fusi, F. Cheli, R. Rebucci

Italian Journal of Animal Science

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Abstract

Abstract Ochratoxin A is a mycotoxin responsible for disease states in both humans and animals. OTA mechanisms of action are numerous, including lipid peroxidation. Oxidative damage results in the modification of macromolecules (i.e. DNA), cell death and tissue injure. Several strategies, such as the use of antioxidants, have been used to reduce OTA cytotoxicity. The aim of this study was to evaluate the role of alpha-toco in counteracting DNA damage induced by OTA in cell cultures. Primary porcine fibroblasts, pherol isolated from embryo and from ear, were incubated for 24h with several concentrations of OTA in order to detect DNA fragmentation. OTA produced DNA fragmentation in a concentration dependent manner in both primary cell cultures. The pre-treatment with alpha-tocopherol caused the reduction of DNA fragmentation in both primary cell cultures, after 24h of incubation with OTA. In particular, when OTA was added at 10 μg/ml in embryo fibroblasts, alpha-tocopherol at the concentrations of 1 nM was significantly (P<0.05) able to reduce DNA fragmentation by 16%. In ear fibroblast cultures, alpha-tocopherol at the 1nM concentration was significantly (P<0.05) able to reduce DNA fragmentation by 15.23% in the presence of 5 μg/ml of OTA.

Study Snapshot

Key takeawayAlpha-tocopherol effectively reduces DNA fragmentation caused by Ochratoxin A in primary porcine fibroblasts, potentially offering a potential treatment for ochratoxin A-induced diseases.

PopulationOlder adults (50-71 years)

Sample size24

MethodsObservational

OutcomesBody Mass Index projections

ResultsSocial networks mitigate obesity in older groups.

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Paper

Role of alpha-tocopherol in counteracting DNA damage induced by Ochratoxin A in primary porcine fibroblasts

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References

Evaluation of the damage induced by ochratoxin A and the protective role of α-tocopherol in cultured bovine mammary epithelial cells

-tocopherol protects against ochratoxin A-induced damage in cultured bovine mammary epithelial cells, suggesting its potential as a natural remedy for ovarian cancer.

Molecular mechanism of α-tocopherol action

-tocopherol has a nonantioxidant molecular function, acting as a ligand for proteins that regulate signal transduction and gene expression, rather than protecting against oxidative damage.

Vitamin E, antioxidant and nothing more.

Alpha-tocopherol's main function is as a peroxyl radical scavenger, maintaining the integrity of long-chain polyunsaturated fatty acids in cell membranes and their bioactivity.

Toxicokinetics and toxicodynamics of ochratoxin A, an update.

Ochratoxin A (OTA) is a mycotoxin that causes nephrotoxicity, hepatotoxicity, teratogenic, and immunotoxic effects in various animal species, with varying toxicokinetic patterns and unclear biotransformation processes.

Ochratoxin A: The Continuing Enigma

Ochratoxin A (OTA) toxicity varies species- and sex-specifically, with potential mechanisms including genotoxic or epigenetic mechanisms, and requires further investigation for safe legal limits in foodstuffs and animal fodder.

Citations

Effects of Ochratoxin A exposure on DNA damage in porcine granulosa cells in vitro.

Ochratoxin A exposure increases DNA damage in porcine granulosa cells, inhibiting their proliferation and affecting DNA repair-related biological processes.

Fluorescence spectroscopic investigation of competitive interactions between ochratoxin A and 13 drug molecules for binding to human serum albumin.

Indometacin, phenylbutazone, warfarin, and furosemide show the highest competitive capacity to displace ochratoxin A from human serum albumin, potentially reducing its toxicity.

Qualitative Behavioural Assessment of emotionality in pigs

QBA effectively assesses emotionality in pigs, distinguishing between manipulated and unmanipulated groups, and can accurately distinguish between positive and negative emotional states.